New Researches in Biotechnology - Facultatea de Biotehnologii ...

More documents

Recommendations

Info

Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011Monasnicotinate D (4). Yellow oil. UV λ max (MeOH): 245, 271, 328. IR ν max(near): 1665, 1716 cm -1 (C=0) ESI-MS: 382 ([M+Na] 4 ). HR-ESI-MS: 382,1994 ([M+Na] + ,C 22 H 29 NaNO 4 + ; calc. 382,1994).Fig. 2 General formula of monasnicotinates [7]Another researchers identified in acetone extract which contains orange azaphylones,another 4 compounds, presented in figure 3, named monaphilol A-D, compounds whichgive the florescence.Biological evaluation indicated that compounds 1–4 inhibited nitric oxide (NO) productionon lipopolysaccharide-stimulated RAW 264.7 cells. Compounds 1–4 also exhibitedantiproliferative activities against human laryngeal carcinoma (HEp-2) and human colonadenocarinoma (WiDr).Main characteristics of monaphilols is presented in the followingsection.25Monaphilol A (1): orange dark oil; [α] D = 2648,8° (c= 1,23, acetone); UV(MeOH) λ max (log ε): 469 (4,0), 302 (3,4); IR ν max (KBr) 3401, 2954, 2918, 2859, 1742,1655, 1528, 1437, 1239, 1168, 1073, 902, 827 cm -1 ; ESIMS m/z385 [M + H] + ; HRESIMSm/z385.2016 [M + H] + (calc. 385.2015; C 23 H 29 O s ).2. MATERIAL AND METHODSThe aims of the paper is to evaluate the potential therapeutic effect of e Monascussp.metabolited (by antioxidant and cicatrisation study).Monascus metabolite extracte wasobtained by dissloved the red powder (obtained in solid state biosynthesis betwenMonascus sp. and on rice) in ethanol.Materials. The luminol (LH 2 ) - hydrogen peroxide (H 2 O 2 ) system withconcentration of LH 2 = 2.5 x 10 –5 M and H 2 O 2 = 30 mM in Tris-HCl buffer, pH 8.10, wasconsidered as reference system. The Monascus metabolites (10% collagen in water and50% Monascus metabolites) was diluted in water and ethanol of spectroscopic grade; Thestock solution was: 200 µl pigment at 3 ml of water and ethanol, respectively.Methods and apparatus. Absorption spectra have been performed with a PerkinElmer Lambda 35 Spectrometer. The chemiluminescence measurements have been150
Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011performed with a TD 20/20 chemiluminometer Turner Design. Measurements were carriedout in five replicas and averaged, obtaining a relative scattering of the results of up to 10%of the average value; The working volume was 1000 µl.The antioxidant activity of thepigment, A %, was calculated according to the equation:A (%) = ( I I)0−I0× 100 (1)Fig.3Structure of monaphiloles A-D [8].Monaphilol B (2): orange dark oil[α] D 25 =2581,8° (c=0,33, acetona); UV(MeOH λ max (log ε): 469 (4.0), 303 (3,4);IR ν max (KBr) 3398, 2958, 2930, 2851,1734, 1655, 1524, 1441, 1263, 1239, 1172,1073, 906, 796 cm -1 . ESIMS m/z 357 [M +H] + ; HRESIMS m/z 357.1698 [M + H] +(calc. 357.1702; C 21 H 2S O s ).Monaphilol C (3): orange dark25oil; [α] D = 2095,7° (c= 3,49, acetona);UV (MeOH) λ max (log ε): 470 (3.9), 307(3.4); IR ν max (KBr) 3366, 2958, 2926,2855, 1746, 1663, 1528, 1449, 1358, 1239,1073, 958, 835 cm -1 ; ESIMS m/z441 [M +H] + ; HRESIMS m/z441.22S6 [M + H] +(calc. 441,2277; C 26 H 33 O 6 ).Monaphilol D (4): orange dark oil25[α] D = 1491,1° (c= 0,45, acetona); UV(MeOH, λ max (log ε:) 470 (4,0), 307 (3,4);IR ν max (KBr) 3366, 2958, 2926, 2859,1738, 1667, 1528, 1441, 1362, 1235, 1168,1069, 891,807 cm -1 ; ESIMS m/z 413 [M +H] + ; HREIMS m/z 413.1997 [M + H] +(calc. 413,1964; C 24 O 29 H 6 ).Where I 0 and I represent CL intensity measured for the reference system and for thereference system in the presence of the pigment, respectively. Both values were measured5s after the beginning of the reaction. The fluorescence emission spectra of Monascusmetabolites were recorded with Jasco FP-6500 Spectrofluorometer.Cicatrisation effect was investigated in vivo,on Wistar mice using a Monascus extractconditioned with IPM (isopropyl myristate)151
Page 1 and 2:
UNIVERSITY OF AGRONOMICAL SCIENCES
Page 3 and 4:
Proceeding of the 4 rd Internationa
Page 5 and 6:
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87 and 88:
Page 89 and 90:
Page 91 and 92:
Page 93 and 94:
Page 95 and 96:
Page 97 and 98:
Page 99 and 100: Proceeding of the 4 rd Internationa
Page 149: Proceeding of the 4 rd Internationa
Page 163 and 164: Day 7Day 10Proceeding of the 4 rd I
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
show all

New Researches in Biotechnology - Facultatea de Biotehnologii ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?