New Researches in Biotechnology - Facultatea de Biotehnologii ...
New Researches in Biotechnology - Facultatea de Biotehnologii ... New Researches in Biotechnology - Facultatea de Biotehnologii ...
Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011Monasnicotinate D (4). Yellow oil. UV λ max (MeOH): 245, 271, 328. IR ν max(near): 1665, 1716 cm -1 (C=0) ESI-MS: 382 ([M+Na] 4 ). HR-ESI-MS: 382,1994 ([M+Na] + ,C 22 H 29 NaNO 4 + ; calc. 382,1994).Fig. 2 General formula of monasnicotinates [7]Another researchers identified in acetone extract which contains orange azaphylones,another 4 compounds, presented in figure 3, named monaphilol A-D, compounds whichgive the florescence.Biological evaluation indicated that compounds 1–4 inhibited nitric oxide (NO) productionon lipopolysaccharide-stimulated RAW 264.7 cells. Compounds 1–4 also exhibitedantiproliferative activities against human laryngeal carcinoma (HEp-2) and human colonadenocarinoma (WiDr).Main characteristics of monaphilols is presented in the followingsection.25Monaphilol A (1): orange dark oil; [α] D = 2648,8° (c= 1,23, acetone); UV(MeOH) λ max (log ε): 469 (4,0), 302 (3,4); IR ν max (KBr) 3401, 2954, 2918, 2859, 1742,1655, 1528, 1437, 1239, 1168, 1073, 902, 827 cm -1 ; ESIMS m/z385 [M + H] + ; HRESIMSm/z385.2016 [M + H] + (calc. 385.2015; C 23 H 29 O s ).2. MATERIAL AND METHODSThe aims of the paper is to evaluate the potential therapeutic effect of e Monascussp.metabolited (by antioxidant and cicatrisation study).Monascus metabolite extracte wasobtained by dissloved the red powder (obtained in solid state biosynthesis betwenMonascus sp. and on rice) in ethanol.Materials. The luminol (LH 2 ) - hydrogen peroxide (H 2 O 2 ) system withconcentration of LH 2 = 2.5 x 10 –5 M and H 2 O 2 = 30 mM in Tris-HCl buffer, pH 8.10, wasconsidered as reference system. The Monascus metabolites (10% collagen in water and50% Monascus metabolites) was diluted in water and ethanol of spectroscopic grade; Thestock solution was: 200 µl pigment at 3 ml of water and ethanol, respectively.Methods and apparatus. Absorption spectra have been performed with a PerkinElmer Lambda 35 Spectrometer. The chemiluminescence measurements have been150
Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011performed with a TD 20/20 chemiluminometer Turner Design. Measurements were carriedout in five replicas and averaged, obtaining a relative scattering of the results of up to 10%of the average value; The working volume was 1000 µl.The antioxidant activity of thepigment, A %, was calculated according to the equation:A (%) = ( I I)0−I0× 100 (1)Fig.3Structure of monaphiloles A-D [8].Monaphilol B (2): orange dark oil[α] D 25 =2581,8° (c=0,33, acetona); UV(MeOH λ max (log ε): 469 (4.0), 303 (3,4);IR ν max (KBr) 3398, 2958, 2930, 2851,1734, 1655, 1524, 1441, 1263, 1239, 1172,1073, 906, 796 cm -1 . ESIMS m/z 357 [M +H] + ; HRESIMS m/z 357.1698 [M + H] +(calc. 357.1702; C 21 H 2S O s ).Monaphilol C (3): orange dark25oil; [α] D = 2095,7° (c= 3,49, acetona);UV (MeOH) λ max (log ε): 470 (3.9), 307(3.4); IR ν max (KBr) 3366, 2958, 2926,2855, 1746, 1663, 1528, 1449, 1358, 1239,1073, 958, 835 cm -1 ; ESIMS m/z441 [M +H] + ; HRESIMS m/z441.22S6 [M + H] +(calc. 441,2277; C 26 H 33 O 6 ).Monaphilol D (4): orange dark oil25[α] D = 1491,1° (c= 0,45, acetona); UV(MeOH, λ max (log ε:) 470 (4,0), 307 (3,4);IR ν max (KBr) 3366, 2958, 2926, 2859,1738, 1667, 1528, 1441, 1362, 1235, 1168,1069, 891,807 cm -1 ; ESIMS m/z 413 [M +H] + ; HREIMS m/z 413.1997 [M + H] +(calc. 413,1964; C 24 O 29 H 6 ).Where I 0 and I represent CL intensity measured for the reference system and for thereference system in the presence of the pigment, respectively. Both values were measured5s after the beginning of the reaction. The fluorescence emission spectra of Monascusmetabolites were recorded with Jasco FP-6500 Spectrofluorometer.Cicatrisation effect was investigated in vivo,on Wistar mice using a Monascus extractconditioned with IPM (isopropyl myristate)151
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Proceed<strong>in</strong>g of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011Monasnicot<strong>in</strong>ate D (4). Yellow oil. UV λ max (MeOH): 245, 271, 328. IR ν max(near): 1665, 1716 cm -1 (C=0) ESI-MS: 382 ([M+Na] 4 ). HR-ESI-MS: 382,1994 ([M+Na] + ,C 22 H 29 NaNO 4 + ; calc. 382,1994).Fig. 2 General formula of monasnicot<strong>in</strong>ates [7]Another researchers i<strong>de</strong>ntified <strong>in</strong> acetone extract which conta<strong>in</strong>s orange azaphylones,another 4 compounds, presented <strong>in</strong> figure 3, named monaphilol A-D, compounds whichgive the florescence.Biological evaluation <strong>in</strong>dicated that compounds 1–4 <strong>in</strong>hibited nitric oxi<strong>de</strong> (NO) productionon lipopolysacchari<strong>de</strong>-stimulated RAW 264.7 cells. Compounds 1–4 also exhibitedantiproliferative activities aga<strong>in</strong>st human laryngeal carc<strong>in</strong>oma (HEp-2) and human colona<strong>de</strong>nocar<strong>in</strong>oma (WiDr).Ma<strong>in</strong> characteristics of monaphilols is presented <strong>in</strong> the follow<strong>in</strong>gsection.25Monaphilol A (1): orange dark oil; [α] D = 2648,8° (c= 1,23, acetone); UV(MeOH) λ max (log ε): 469 (4,0), 302 (3,4); IR ν max (KBr) 3401, 2954, 2918, 2859, 1742,1655, 1528, 1437, 1239, 1168, 1073, 902, 827 cm -1 ; ESIMS m/z385 [M + H] + ; HRESIMSm/z385.2016 [M + H] + (calc. 385.2015; C 23 H 29 O s ).2. MATERIAL AND METHODSThe aims of the paper is to evaluate the potential therapeutic effect of e Monascussp.metabolited (by antioxidant and cicatrisation study).Monascus metabolite extracte wasobta<strong>in</strong>ed by dissloved the red pow<strong>de</strong>r (obta<strong>in</strong>ed <strong>in</strong> solid state biosynthesis betwenMonascus sp. and on rice) <strong>in</strong> ethanol.Materials. The lum<strong>in</strong>ol (LH 2 ) - hydrogen peroxi<strong>de</strong> (H 2 O 2 ) system withconcentration of LH 2 = 2.5 x 10 –5 M and H 2 O 2 = 30 mM <strong>in</strong> Tris-HCl buffer, pH 8.10, wasconsi<strong>de</strong>red as reference system. The Monascus metabolites (10% collagen <strong>in</strong> water and50% Monascus metabolites) was diluted <strong>in</strong> water and ethanol of spectroscopic gra<strong>de</strong>; Thestock solution was: 200 µl pigment at 3 ml of water and ethanol, respectively.Methods and apparatus. Absorption spectra have been performed with a Perk<strong>in</strong>Elmer Lambda 35 Spectrometer. The chemilum<strong>in</strong>escence measurements have been150
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