New Researches in Biotechnology - Facultatea de Biotehnologii ...

Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011The length of these rods and vibrio obtained by transmission electron microscopymeasurements is likely to exceed 0.5 µm.In Figure 4 are the few examples of measurements performed by TEM on bacteria withinthe microcosms. In microcosm 2 at zero time average cell length was 0.800 µm, but after24 hours incubation, the average cell length was 2.250 µm; however in M4 where there wasan additional nitrogen source, the average cell length was 4.750 µm at T 6 .Figure 4. Bacterial cell size in M2 and M4 measured by TEM at T 0 (0 hours) and T 6 (24 hours).The experimental TEM results illustrate the presence of granules (Figure 5) in analyzedcells.144Figure 5. Section in the cell wall (x 145.000). The arrows indicate small grains observed at thesurface and inside the cell.It is difficult to interpret the electron diffraction for a particular crystalline phase. In M4sample there is the possibility that the grains could be polyhydroxybutyrate (PHB) becauseof the presence of the crystalline phase with orthorhombic structure, with network