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Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011Fig. 7. Small leaflets of C. davidovii formed on a leaf explant on the C7 medium(left) and growing explants transferred to the MS medium (right).Table 2. Response of the explants to the different media (C - callus; R - roots; IS - indirect shootformation; DS - direct shoot formation).Species Centaurea caliacrae Centaurea davidoviiMeidumType ofexplantLeafsegmentsRootsegmentsWholeplantletsMS C, DS C, IS C, RC1 C, R, DS C, IS CC2 C C, R CC3 C, R R, DS C, RC4C5LeafsegmentsC6 C, IS CCCRootsegmentsC7 C, IS C, ISC8C, ISCC1 C CCC2 C CCG C (necrosis) C (necrosis)4. CONCLUSIONSuccessful in vitro germination of seeds of three endemic species – Centaurea caliacrae, C.davidovii and C. finazzeri – was demonstrated. The germination rate of C. caliacrae seedswas high regardless of treatment with giberellin. The plants grew well in vitro on thephytohormone-free MS medium and could be used as an explant source for furtherexperiments, as well as for ex vitro adaptation, which proved to be easy, with a highsurvival rate. The PGR-supplemented media used stimulated mainly callus formation andsome shoot growth, especially when using the medium containing 0,5 mg/l BAP. The138
Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011formation of a callus-like tissue in an untreated seedling of C. davidovii was observed alongwith strong stem and root growth and formation of adventitious stems. The furthercollection of seeds will make it possible to clarify the frequency of this event and willprovide plant material for future research.REFERENCESBancheva, S., Gorgorov, R.. (2010): Taxonomic revision and conservation status of Centaureadavidovii (sect. Lepteranthus, Asteraceae). Phytologia Balcanica 16(2): 255-261.Cuenca, S., Amo-Marco, J. B., Parra, R. (1999): Micropropagation from inflorescence stems of theSpanish endemic plant Centaurea paui Loscos ex Willk. (Compositae). Plant Cell Reports 18: 674–679.Hammatt, N., Evans, P. K. (1985): The in vitro propagation of an endangered species: Centaureajunoniana Svent. (Compositae). Journal of Horticultural Science 60 (1) 93-97.Kumar, J. V., Ranjitha Kumari, B. D., Castaño, E. (2008): Cyclic somatic embryogenesis andefficient plant regeneration from callus of safflower. Biologia Plantarum 52 (3): 429-436.Murashige, T., Skoog, F., (1962): A revised medium for rapid growth and bioassays with tobaccocultures. Physiol. Plant 15: 473-497.Peev, D. (Editor) (2011) Red Data Book of Bulgaria, Vol. 1 – Plants. Online version:http://e-ecodb.bas.bg/rdb/bg/vol1/Robinson J. P., Britto S. J., Balakrishnan, V. (2009): Regeneration of plants through somaticembryogenesis in Emilia zeylanica C. B. Clarke - a potential medicinal herb. Botany ResearchInternational 2 (1): 36-41.Wildi, E., Schaffner, W., Berger Büter, K. (1998): In vitro propagation of Petasites hybridus(Asteraceae) from leaf and petiole explants and from inflorescence buds. Plant Cell Reports 18: 336–340.139
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