New Researches in Biotechnology - Facultatea de Biotehnologii ...

Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011Fig. 7. Small leaflets of C. davidovii formed on a leaf explant on the C7 medium(left) and growing explants transferred to the MS medium (right).Table 2. Response of the explants to the different media (C - callus; R - roots; IS - indirect shootformation; DS - direct shoot formation).Species Centaurea caliacrae Centaurea davidoviiMeidumType ofexplantLeafsegmentsRootsegmentsWholeplantletsMS C, DS C, IS C, RC1 C, R, DS C, IS CC2 C C, R CC3 C, R R, DS C, RC4C5LeafsegmentsC6 C, IS CCCRootsegmentsC7 C, IS C, ISC8C, ISCC1 C CCC2 C CCG C (necrosis) C (necrosis)4. CONCLUSIONSuccessful in vitro germination of seeds of three endemic species – Centaurea caliacrae, C.davidovii and C. finazzeri – was demonstrated. The germination rate of C. caliacrae seedswas high regardless of treatment with giberellin. The plants grew well in vitro on thephytohormone-free MS medium and could be used as an explant source for furtherexperiments, as well as for ex vitro adaptation, which proved to be easy, with a highsurvival rate. The PGR-supplemented media used stimulated mainly callus formation andsome shoot growth, especially when using the medium containing 0,5 mg/l BAP. The138