New Researches in Biotechnology - Facultatea de Biotehnologii ...

More documents

Recommendations

Info

Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 201113. Regodón, J.A., Pérez, F., Valdés, M.E., De Miguel, C., Ramírez, M. (1997): A simple andeffective procedure for selection of wine yeast strains. Food Microbiol. Vol. 14, 3:247–254.14. White, J. (1954): Yeast Technology. John Wiley and Sons, New York.15. xxx, (2000). Official Methods of Analysis of AOAC International, 17th Edition. Edited byWilliam Horwitz, AOAC International.16. x x x (2002). Manualul inginerului de industrie alimentara, Bucuresti, Editura Tehnica, Vol. II,1414-1420.17. x x x (1992): Assessment of VOC emissions and their control from baker's yeast manufacturingfacilities, prepared by Midwest Research Institute for the United States Environmental ProtectionAgency, Office of Air Quality Planning and Standards, Control Technology Center, 7-17.18. http://www.agroinfo.ro/vegetal/vesti-bune-pentru-procesatorii-de-sfecla-de-zahar.19. http://www.capital.ro/detalii-articole/stiri/romania-isi-poate-asigura-necesarul-de-zahar-darproduce-numai-o-cincime-din-potential-151510.html.132
Proceeding of the 4 rd International Symposium“NEW RESEARCH IN BIOTECHNOLOGY” USAMV Bucharest, Romania, 2011IN VITRO CULTURES OF BALKAN AND BULGARIAN ENDEMICCENTAUREA SPECIESROSSEN GORGOROV *,1 , MARINA STANILOVA * , SVETLANA BANCHEVA ** Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences23, Acad. G. Bonchev Str., 1113 Sofia, Bulgaria1 Corresponding author: E-mail: rossen6540@yahoo.comAbstract. Centaurea caliacrae and C. finazzeri are Balkan endemic species and C. davidovii isendemic for Bulgaria. In this study, in vitro cultures of the three species were initiated from seeds asa means of ex situ conservation. Due to the species’ conservation status, the available plant materialwas limited, especially with C. davidovii. An average of 90% of the seeds of C. caliacrae germinatedon MS nutrient medium in the first 20 days of cultivation regardless of phytohormone treatment(giberellic acid). Eleven MS-based media were used with different combinations and concentrationsof cytokinins and auxins. Green callus formation and occasional organogenesis were the mostfrequent response to plant growth regulators. A few adventitious shoots were formed by cultivatedstems, especially on the medium supplemented with 0,5 g/l benzylaminopurine. On the MS medium, C.caliacrae plants grew well, rooted and were successfully transferred to ex vitro conditions with asurvival rate of 84%. A single C. davidovii seedling on phytohormone-free MS medium formedadventitious stems and a callus-like tissue at the base and originated a clone, suitable for furtherexperiments with uniform plant material.On the medium supplemented with 3,5 mg/l thidiazuron and1,4 mg/l benzylaminopurine, leaf explants formed new leaf clusters from callus which continuedgrowing when transferred to the MS medium.Keywords: knapweed, ex situ conservation, plant growth regulators1. INTRODUCTIONThe genus Centaurea s. l. is represented in the Bulgarian flora by more than 70 species andis among the plant groups whose center of speciation includes the territory of Bulgaria. It isalso the genus richest in endemics in the Bulgarian flora (ca. 30%). In the new edition ofthe Red Data Book of Bulgaria (plants) (2011), 14 species are included (ca. 23%). Some ofthe Centaurea species are rare and are represented by one or few populations. This suggestsmeasures for their conservation to be taken, both in situ and ex situ, especially consideringthe reproductive problems those species encounter. Their seeds have a generally lowgermination rate and are very often damaged by insects before being dispersed. In thisstudy, in vitro culture of three endemic species was initiated as a method for ex situconservation – Centaurea caliacrae Prodán, Centaurea davidovii Urumov and Centaureafinazzeri Adamovič. C. caliacrae is a Balkan endemic, occurring along the Black sea coast.C. davidovii is a Bulgarian endemic, occurring only in Stara planina mountain, particularlyin the Central Balkan national park. Its populations are decreasing in size (Bancheva &Gorgorov 2010). C. finazzeri is also a Balkan endemic, represented in Bulgaria by only onepopulation in the Valley of Struma River (Northern part). In addition to in situ conservationmeasures, such as establishment of protected territories and habitat conservation, ex situmethods are also advisable, including in vitro cultivation and micropropagation of the133
Page 1 and 2:
UNIVERSITY OF AGRONOMICAL SCIENCES
Page 3 and 4:
Proceeding of the 4 rd Internationa
Page 5 and 6:
Page 7 and 8:
Page 9 and 10:
Page 11 and 12:
Page 13 and 14:
Page 15 and 16:
Page 17 and 18:
Page 19 and 20:
Page 21 and 22:
Page 23 and 24:
Page 25 and 26:
Page 27 and 28:
Page 29 and 30:
Page 31 and 32:
Page 33 and 34:
Page 35 and 36:
Page 37 and 38:
Page 39 and 40:
Page 41 and 42:
Page 43 and 44:
Page 45 and 46:
Page 47 and 48:
Page 49 and 50:
Page 51 and 52:
Page 53 and 54:
Page 55 and 56:
Page 57 and 58:
Page 59 and 60:
Page 61 and 62:
Page 63 and 64:
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82: Proceeding of the 4 rd Internationa
Page 131: Proceeding of the 4 rd Internationa
Page 163 and 164: Day 7Day 10Proceeding of the 4 rd I
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Page 199 and 200:
Page 201 and 202:
Page 203 and 204:
Page 205 and 206:
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
show all

New Researches in Biotechnology - Facultatea de Biotehnologii ...

Create successful ePaper yourself

Delete template?

Save as template?