FGF-signalling in the differentiation of mouse ES cells towards ...

Figure 4: FGFs activating FGFRc-isoforms affect early cell growth and proliferation. A wt mES cell line (E14)was grown in media containing activin w/wo FGFs and harvested for analysis of total cell number and proliferationon days 3 &5. A count of total number of cells and relative proliferation of cells is shown for day 3 A) and day 5 B).The mean expression ± S.E.M. of 3 independent experiments is shown, using a Student’s t-test for the statisticalanalysis: * = P < 0,05; ** = P < 0,01 compared to the activin conditions.Opposite, Figure 5: FGF4-signalling is dispensable for endoderm differentiation. E14, FGF4 +/– and FGF4 –/– cellswere stained for markers of pluripotency and endoderm. A) Undifferentiated cells were stained for OCT4 andSOX17 and the nuclear stain DAPI. B) Cells were differentiated for 5 days in 30 ng/ml activin w/wo 5 ng/ml FGF4and stained for OCT4 and endoderm markers SOX17, FOXA2, E-cadherin (Ecad) and DAPI. Representativeimages are shown for each condition. Scale bar: 100 µm.78