FGF-signalling in the differentiation of mouse ES cells towards ...

Figure 3: Activation of FGFRb or FGFRc-isoforms differentially affects the expression of PS, DE andmesoderm markers. Sox17-GFP cells were differentiated in media containing BMP4 or activin w/wo FGFs for 3-5days before harvest. Cells were stained for markers of PS, DE or mesoderm and analysed using a FACS. A & B)Cells were stained for T and analysed on days 3 and 5. Shown here are the relative number of T + cells in the Sox17-GFP Hi fraction A), or the Sox17-GFP –/Lo fraction B). The mean expression ± S.E.M. of 2 independent experimentsare shown, no statistical analysis performed. C-G) Cells were stained for FLK1 and EpCAM and analysed in eithersingle channel for C) Sox17-GFP, D) FLK1, E) EpCAM; or in multichannel for the Sox17-GFP Hi fraction F), orSox17-GFP –/Lo fraction G) dividing data into four fractions: FLK1 – /EpCAM + ; FLK1 + /EpCAM + ; FLK1 – /EpCAM – ;FLK1 + /EpCAM – . The mean expression ± S.E.M. of 3 independent experiments is shown, using a Student’s paired,two-tailed t-test for the statistical analysis: * = P < 0,05; ** = P < 0,01 compared to the activin conditions.77