FGF-signalling in the differentiation of mouse ES cells towards ...
FGF-signalling in the differentiation of mouse ES cells towards ...
FGF-signalling in the differentiation of mouse ES cells towards ...
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FiguresFigure 1: Screen for <strong>FGF</strong>R-isotypes dur<strong>in</strong>g DE <strong>differentiation</strong> <strong>in</strong> sorted fractions <strong>of</strong> Sox17-GFP <strong>cells</strong>. Theexpression <strong>of</strong> each <strong>FGF</strong>R-is<strong>of</strong>orm was analysed by qPCR <strong>in</strong> both sorted and unsorted fractions <strong>of</strong> Sox17-GFP <strong>cells</strong>,differentiated by our DE protocol (30 ng/ml activ<strong>in</strong> for 5 days). A) A histogram show<strong>in</strong>g sort<strong>in</strong>g gates <strong>in</strong> GFP – ,GFP Lo and GFP Hi fractions. B) The absolute expression <strong>of</strong> each <strong>FGF</strong>R-is<strong>of</strong>orm was standardised to <strong>the</strong> housekeep<strong>in</strong>ggene TATA-b<strong>in</strong>d<strong>in</strong>g prote<strong>in</strong> (Tbp). Sox17 Hi fractions are shown only at day 4 and 5, when <strong>the</strong>y appeared <strong>in</strong><strong>the</strong> culture. The fraction <strong>of</strong> Sox17-GFP – <strong>cells</strong> was to low for RNA-extraction. We did not obta<strong>in</strong> functional primersfor <strong>FGF</strong>R3b. The relative mean expression ± S.E.M. <strong>of</strong> 3 <strong>in</strong>dependent experiments is shown, us<strong>in</strong>g a Student’spaired, two-tailed t-test for <strong>the</strong> statistical analysis: * = P < 0,05; ** = P < 0,01 compared to <strong>the</strong> <strong>ES</strong>C condition foreach fraction (Sox17-GFP + fractions were compared to <strong>the</strong> unsorted <strong>ES</strong>C sample).75
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