FGF-signalling in the differentiation of mouse ES cells towards ...

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Ying, Q.L., J. Nichols, I. Chambers, and A. Smith. 2003a. BMP induction of Id proteins suppressesdifferentiation and sustains embryonic stem cell self-renewal in collaboration with STAT3. Cell115: 281-92.Ying, Q.L., M. Stavridis, D. Griffiths, M. Li, and A. Smith. 2003b. Conversion of embryonic stem cellsinto neuroectodermal precursors in adherent monoculture. Nat Biotechnol 21: 183-6.Zhang, X., O.A. Ibrahimi, S.K. Olsen, H. Umemori, M. Mohammadi, and D.M. Ornitz. 2006. Receptorspecificity of the fibroblast growth factor family. The complete mammalian FGF family. J BiolChem 281: 15694-700.74
FiguresFigure 1: Screen for FGFR-isotypes during DE differentiation in sorted fractions of Sox17-GFP cells. Theexpression of each FGFR-isoform was analysed by qPCR in both sorted and unsorted fractions of Sox17-GFP cells,differentiated by our DE protocol (30 ng/ml activin for 5 days). A) A histogram showing sorting gates in GFP – ,GFP Lo and GFP Hi fractions. B) The absolute expression of each FGFR-isoform was standardised to the housekeepinggene TATA-binding protein (Tbp). Sox17 Hi fractions are shown only at day 4 and 5, when they appeared inthe culture. The fraction of Sox17-GFP – cells was to low for RNA-extraction. We did not obtain functional primersfor FGFR3b. The relative mean expression ± S.E.M. of 3 independent experiments is shown, using a Student’spaired, two-tailed t-test for the statistical analysis: * = P < 0,05; ** = P < 0,01 compared to the ESC condition foreach fraction (Sox17-GFP + fractions were compared to the unsorted ESC sample).75
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PhD thesisCand.scient. Janny Marie
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ResuméSukkersyge er en sygdom der
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Table of contents1
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ICMinner cell massIdInhibitor of di
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cell mass regenerates probably thro
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Figure 1-1: Early embryo developmen
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Figure 1-3: Regional expression of
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The pluripotent stateThe pluripoten
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There are four membrane-bound FGFRs
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2. AimsThe aim of this study was to
Page 30 and 31: Developmental Biology 330 (2009) 28
Page 32 and 33: 288 M. Hansson et al. / Development
Page 50 and 51: Figure S2Figure S2: A subpopulation
Page 52 and 53: Figure S4Figure S4: Expression of T
Page 54 and 55: Figure S6Figure S6: qRT-PCR analyse
Page 56 and 57: epithelium; Cdx2, expressed posteri
Page 58 and 59: Figure 4-4: A high FGF4-concentrati
Page 60 and 61: Figure 4-6: A 3-step protocol does
Page 63 and 64: 5. Paper IIFGFR(IIIc)-activation in
Page 65 and 66: AbstractProgress in embryonic stem
Page 67 and 68: variants in their Ig-like domain II
Page 69 and 70: Cell count and proliferation assayC
Page 71 and 72: influence on the numbers of Sox17-G
Page 73 and 74: undifferentiated cells, we found th
Page 75 and 76: FGF4, 5, FGF8b and FGFR1, are expre
Page 77 and 78: with EdU-stain (blue sample); and w
Page 79: Olsen, S.K., J.Y. Li, C. Bromleigh,
Page 83 and 84: Figure 3: Activation of FGFRb or FG
Page 87 and 88: Opposite, Figure 6: In the absence
Page 89 and 90: 6. General discussionEndoderm diffe
Page 91 and 92: Overall, the multitude of FGF-signa
Page 93 and 94: transplantation is the spread of an
Page 96: AcknowledgementsThe work presented
Page 99 and 100: Chambers, I., D. Colby, M. Robertso
Page 101 and 102: Hawkins, V.J. Wroblewski, D.S. Li,
Page 103 and 104: Nishikawa, S.I., S. Nishikawa, M. H
Page 105 and 106: Tanimizu, N., H. Saito, K. Mostov,
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