FGF-signalling in the differentiation of mouse ES cells towards ...
FGF-signalling in the differentiation of mouse ES cells towards ...
FGF-signalling in the differentiation of mouse ES cells towards ...
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Figure S3Figure S3: <strong>ES</strong> cell progeny treated with activ<strong>in</strong> express markers <strong>of</strong> def<strong>in</strong>itive endoderm and not visceralendoderm. (A) Flow cytometric analysis <strong>of</strong> CXCR4 expression <strong>in</strong> Mixl1 Gfp/+ <strong>cells</strong> grown for 5 days with or without100 ng/ml activ<strong>in</strong>. Mouse embryonic day (E)11 head tissue was used as control. (B, C) RT-PCR analysis for Sox7expression <strong>in</strong> T Gfp/+ <strong>cells</strong> (B) and Sox17, Pyy, Sox7 and Tdh expression <strong>in</strong> Flk1 LacZ/+ <strong>cells</strong> (C). Cells were cultured <strong>in</strong>0, 3 or 100 ng/ml activ<strong>in</strong>, 10 ng/ml BMP4, 100 ng/ml Wnt3a or 100 ng/ml activ<strong>in</strong> + 100 ng/ml Wnt3a for 5 days as<strong>in</strong>dicated. E7 <strong>mouse</strong> embryo cDNA was used as positive control and expression <strong>of</strong> Tbp and G6pd was analyzed asreference genes.45