FGF-signalling in the differentiation of mouse ES cells towards ...

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Figure S2Figure S2: A subpopulation of T + cells expresses Mixl1, whereas Gsc + cells express no or low levels of T.Immunofluorescent analyses of T expression in T Gfp/+ (A), Mixl1 Gfp/+ (B) or Gsc Gfp/+ cells (C) grown in adherentculture for 3 or 5 days in the presence of the indicated growth factors. The arrows in (C) indicate areas with high Texpression and low/no Gsc (GFP) expression. (D) Immunofluorescent analyses of Foxa2 and E-cad expression inT Gfp/+ (a–d), Mixl1 Gfp/+ (e–h), or Gsc Gfp/+ cells (i–l) after activin treatment.44
Figure S3Figure S3: ES cell progeny treated with activin express markers of definitive endoderm and not visceralendoderm. (A) Flow cytometric analysis of CXCR4 expression in Mixl1 Gfp/+ cells grown for 5 days with or without100 ng/ml activin. Mouse embryonic day (E)11 head tissue was used as control. (B, C) RT-PCR analysis for Sox7expression in T Gfp/+ cells (B) and Sox17, Pyy, Sox7 and Tdh expression in Flk1 LacZ/+ cells (C). Cells were cultured in0, 3 or 100 ng/ml activin, 10 ng/ml BMP4, 100 ng/ml Wnt3a or 100 ng/ml activin + 100 ng/ml Wnt3a for 5 days asindicated. E7 mouse embryo cDNA was used as positive control and expression of Tbp and G6pd was analyzed asreference genes.45
Page 1: PhD thesisCand.scient. Janny Marie
Page 5: ResuméSukkersyge er en sygdom der
Page 9: Table of contents1
Page 12 and 13: ICMinner cell massIdInhibitor of di
Page 14 and 15: cell mass regenerates probably thro
Page 16 and 17: Figure 1-1: Early embryo developmen
Page 18 and 19: Figure 1-3: Regional expression of
Page 20 and 21: The pluripotent stateThe pluripoten
Page 25 and 26: There are four membrane-bound FGFRs
Page 27: 2. AimsThe aim of this study was to
Page 30 and 31: Developmental Biology 330 (2009) 28
Page 32 and 33: 288 M. Hansson et al. / Development
Page 52 and 53: Figure S4Figure S4: Expression of T
Page 54 and 55: Figure S6Figure S6: qRT-PCR analyse
Page 56 and 57: epithelium; Cdx2, expressed posteri
Page 58 and 59: Figure 4-4: A high FGF4-concentrati
Page 60 and 61: Figure 4-6: A 3-step protocol does
Page 63 and 64: 5. Paper IIFGFR(IIIc)-activation in
Page 65 and 66: AbstractProgress in embryonic stem
Page 67 and 68: variants in their Ig-like domain II
Page 69 and 70: Cell count and proliferation assayC
Page 71 and 72: influence on the numbers of Sox17-G
Page 73 and 74: undifferentiated cells, we found th
Page 75 and 76: FGF4, 5, FGF8b and FGFR1, are expre
Page 77 and 78: with EdU-stain (blue sample); and w
Page 79 and 80: Olsen, S.K., J.Y. Li, C. Bromleigh,
Page 81 and 82: FiguresFigure 1: Screen for FGFR-is
Page 83 and 84: Figure 3: Activation of FGFRb or FG
Page 87 and 88: Opposite, Figure 6: In the absence
Page 89 and 90: 6. General discussionEndoderm diffe
Page 91 and 92: Overall, the multitude of FGF-signa
Page 93 and 94: transplantation is the spread of an
Page 96: AcknowledgementsThe work presented
Page 99 and 100: Chambers, I., D. Colby, M. Robertso
Page 101 and 102:
Hawkins, V.J. Wroblewski, D.S. Li,
Page 103 and 104:
Nishikawa, S.I., S. Nishikawa, M. H
Page 105 and 106:
Tanimizu, N., H. Saito, K. Mostov,
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