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Supplementary data, Paper I‘A late requirement for Wnt and <strong>FGF</strong> signal<strong>in</strong>g ’dur<strong>in</strong>g activ<strong>in</strong>-<strong>in</strong>ducde formation <strong>of</strong>foregut endoderm from <strong>mouse</strong> embryonic stem <strong>cells</strong>’Developmental Biology, 2009, 330, p. 286 – 304.Mattias Hansson, Dor<strong>the</strong> R- Olesen, Janny M.L. Peterslund, N<strong>in</strong>a Engberg, Morten Kahn,Maria W<strong>in</strong>zi, T<strong>in</strong>o Kle<strong>in</strong>, Poul Maddox-Hyttel and Palle SerupFigure S1Figure S1: BMP4 and Activ<strong>in</strong> <strong>in</strong>duction <strong>of</strong> GFP expression <strong>in</strong> T Gfp/+ , Mixl1-Gsc Gfp/+ , Gsc Gfp/+ and Sox17 Gfp/+<strong>cells</strong>. Primary flow cytometry histograms <strong>of</strong> GFP expression <strong>in</strong> T Gfp/+ and Mixl1Gfp/+ <strong>cells</strong> cultured for 3 days withor without 10 ng/ml BMP4 and Gsc Gfp/+ and Sox17 Gfp/+ <strong>cells</strong> cultured for 5 days <strong>in</strong> <strong>the</strong> presence or absence <strong>of</strong>100 ng/ml activ<strong>in</strong>43
- Page 1: PhD thesisCand.scient. Janny Marie
- Page 5: ResuméSukkersyge er en sygdom der
- Page 9: Table of contents1
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- Page 20 and 21: The pluripotent stateThe pluripoten
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- Page 27: 2. AimsThe aim of this study was to
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- Page 50 and 51: Figure S2Figure S2: A subpopulation
- Page 52 and 53: Figure S4Figure S4: Expression of T
- Page 54 and 55: Figure S6Figure S6: qRT-PCR analyse
- Page 56 and 57: epithelium; Cdx2, expressed posteri
- Page 58 and 59: Figure 4-4: A high FGF4-concentrati
- Page 60 and 61: Figure 4-6: A 3-step protocol does
- Page 63 and 64: 5. Paper IIFGFR(IIIc)-activation in
- Page 65 and 66: AbstractProgress in embryonic stem
- Page 67 and 68: variants in their Ig-like domain II
- Page 69 and 70: Cell count and proliferation assayC
- Page 71 and 72: influence on the numbers of Sox17-G
- Page 73 and 74: undifferentiated cells, we found th
- Page 75 and 76: FGF4, 5, FGF8b and FGFR1, are expre
- Page 77 and 78: with EdU-stain (blue sample); and w
- Page 79 and 80: Olsen, S.K., J.Y. Li, C. Bromleigh,
- Page 81 and 82: FiguresFigure 1: Screen for FGFR-is
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- Page 87 and 88: Opposite, Figure 6: In the absence
- Page 89 and 90: 6. General discussionEndoderm diffe
- Page 91 and 92: Overall, the multitude of FGF-signa
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- Page 96: AcknowledgementsThe work presented
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Chambers, I., D. Colby, M. Robertso
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Hawkins, V.J. Wroblewski, D.S. Li,
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Nishikawa, S.I., S. Nishikawa, M. H
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Tanimizu, N., H. Saito, K. Mostov,