ACE LC/MS and Rapid Analysis HPLC Columns - Teknolab AS

Increased Column LifetimeSpecially Manufactured for High Flow ApplicationsTo maximize sample throughput, very high mobile phase linearvelocity (flow rate) is often used, sometimes 3 to 4 times higherthan would be used for typical HPLC applications. This putsextra strain on the column packing bed and causes early failuredue to voids or channels that develop. ACE stationary phasesare mechanically very strong and able to withstand aggressivepacking techniques. This has permitted the development ofproprietary packing technology that is designed specifically toproduce columns robust enough for high throughput applications.FIGURE 7ACE HPLC Columns Are Compatible with a Wide Rangeof "MS Friendly" BufferspH 20.1% TFA1 20.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0Time (min)pH 310mM NH 4 COOH1 20.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0Time (min)pH 710mM NH 4 HCO 31 20.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0Time (min)pH 1010mM NH 4 HCO 310.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0Time (min)pH 10.510mM NH 4 OH0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0Time (min)Column: ACE C18, 50 x 2.1 mm, 3µm, Mobile Phase: 80% MeOH, 20% buffer,Flow Rate: 0.20 ml/min, Temp: 22°C, Sample: 1)Nortriptyline 2) Amitriptyline,Wavelength: 215 nmACE HPLC columns can be used over a broad pH range, allowingfor simplified method development on a single column. And,ACE columns are compatible with volatile mobile phase buffersrecommended for LC/MS applications.122Increased retention of basic moleculesFIGURE 8Columns Specially Manufactured for Long Life Under HighThroughput Conditions% Efficiency100806040Leading Competitor 3µm C80 1000 2000 3000 4000 5000Column:Gradient:Flow Rate:No. of Gradient CyclesACE 3 C850 x 3.0 mmFast gradient cycling, 5% to 95% Acetonitrile in 2.25 minutes1.25 ml/minIn this lifetime test under high flow and fast gradientconditions, the specially manufactured ACE HPLCcolumn showed no loss in performance after over5,000 gradient cycles. A typical "fast analysis"column failed to provide acceptable performanceafter less than a 1,000 gradient cycles.Highly Stable Columns for Robust, Reliable MethodsACE HPLC columns, by virtue of their high bonding densityand ultra-inert characteristics, can be used with mobile phaseconditions that are optimal for LC/MS applications. Stabilityat both low and high pH and compatibility with volatile buffersmakes these the right columns for demanding situations suchas standard methods and open access LC/MS. The proprietarypacking technology used to specially manufacture these columnsfor high throughput applications produces extremely durableHPLC columns that are the key to robust LC/MS methods.SummaryACE Rapid Analysis HPLC Columns are Optimized for HighSample Throughput Applications• High ThroughputACE columns are now available in ultra short column lengthsdesigned for high resolution of complex mixtures in seconds.• High SensitivitySmall-bore columns packed with high efficiency ultra inertstationary phases provide excellent peak shape for maximumsensitivity. For peptide/protein samples, ACE columnsprovide maximum sensitivity, since TFA is not required in themobile phase.• Robust PerformanceProprietary column packing technology produces columnsrugged enough to stand up to the demands of high throughputapplications. Highly inert stationary phases eliminate columnbleed and permit operation over a wide range of mobile phaseconditions, allowing optimal conditions for LC/MS applications.