ACE LC/MS and Rapid Analysis HPLC Columns - Teknolab AS

High Efficiency Short Columns forRapid AnalysisFIGURE 4Using Ace HPLC Columns for Fast Separations150 x 2.1 mm0.2ml/min50 x 2.1 mm0.2ml/min30 x 2.1 mm0.2ml/min20 x 2.1 mm0.2ml/min12 3 40 1 2 3 4 5 6 7 8 9 101Time (min)2 3 40 1 2 3 4 5 6 7 8 9 10Time (min)12 3 40 1 2 3 4 5 6 7 8 9 10Time (min)13 42Increased SensitivityACE HPLC columns, by virtue of their inert characteristics, giveinherently higher sensitivity because of the sharp, narrow peaksproduced by their stationary phases. When there is a limitedamount of sample for analysis, which is often the case in drugdiscovery, sensitivity can be increased by choosing columnswith smaller internal diameters. As the column internaldiameter decreases, the volume of the chromatographicpeaks also decreases, concentrating the sample and increasingsensitivity. Columns with 2.1 mm and 1.0 mm internaldiameters are particularly attractive for situations where theamount of sample is limited but sensitivity is important.FIGURE 5The Effect of Column id on SensitivityColumn id Relative Sensitivity4.6 mm --3.0 mm 2.32.1 mm 4.81.0 mm 21As the column internal diameter decreases, the volume of thechromatographic peaks decreases, concentrating the sampleand increasing sensitivity. If the same amount of sample isinjected, a column with an id of 2.1 mm will give about 5 foldthe sensitivity of a column with an id of 4.6 mm.0 1 2 3 4 5 6 7 8 9 10Time (min)20 x 2.1 mm0.8ml/min1234431 20.0 0.1 0.2 0.3 0.4Time (min)0 1 2 3 4 5 6 7 8 9 10Time (min)FIGURE 6Effect of Tailing on SensitivityColumn: ACE 3 C18Mobile Phase: 80:20 MeOH/H2OSample: 1) dimethyl phthalate2) toluene3) biphenyl4) phenanthreneLeading C8 ColumnACE C8 ColumnACE HPLC columns are now available in new ultra short20mm and 30mm lengths enabling rapid, reproducible highthroughput screening at elevated flow rates.1.4 1.6 1.8 2.0 2.21.4 1.6 1.8 2.0 2.2ACE ultra inert base deactivated HPLC columns virtuallyeliminate the negative effects of silanols in HPLC separations.This unequalled performance is now available in 20, 30, 35 and50 mm length columns, with diameters from 1.0 to 4.6 mm i.d.The ACE columns are suitable for high throughput and LC/MSapplications, and are the ideal choice for high volume screeningassays used for drug analysis and combinatorial libraries whererobust, reproducible columns are essential.Apart from being the most inert HPLC columns on the market,they are also the most efficient, and are manufactured andvalidated to the same exacting standards as all ACE columns.Increased efficiency is an important benefit given the shortcolumns typically used in LC/MS and rapid analysis applications.2.02.0This figure compares the LC/MS signal intensity on twodifferent brands of HPLC columns. Because ACE stationaryphases are more inert, with less silanol interference, thechromatographic peak produced by the ACE C8 column hasless tailing and, therefore, provides greater sensitivity. Thehighly inert characteristics of the ACE HPLC columns givethem inherently higher sensitivity than other columns.