Download - Eppendorf

Advancing scientific research for over 60 yearsThe natural selectionEppendorf Mastercycler ® standard and real-time thermal cyclers

Quality is timelessThe latest in the evolutionEppendorf is an innovator, designing instrumentsthat fit the needs of today’s scientist.The Mastercycler gradient by Eppendorf—still popular today—was the first availablegradient thermal cycler. Today the gradientfunction remains critical for the optimizationof a PCR reaction.The next generation Mastercycler ep is a labfavorite and still one of the fastest cyclersaround. Given this history of innovation,it’s only natural that Eppendorf would nowintroduce the latest in evaporation reductiontechnology—vapo.protect . With a shift tolow volume reactions and faster protocols,the need to safeguard the integrity of yoursample has become increasingly vital.The new Mastercycler pro does just that.Eppendorf has also addressed the needsof real-time PCR. Integrating the advantagesof the Mastercycler ep gradient family,we have developed a fully licensed real-timePCR system that meets the requirements ofthe latest quantitative PCR applications.A history of advanced PCR‡ Mastercycler 5330:Eppendorf introduces the‡ Microcycler: Eppendorffirst Mastercycler based onintroduces its first thermalpeltier technology.cycler using water to heatand cool.19931990‡ Mastercycler gradient: ‡ Thin-walled 0.2 mlthe first gradient thermal PCR tubes allow forcycler on the market.rapid heat transfer.1997 1998

Introducing the Mastercycler proThe proprietary vapo.protect technology reducesevaporation to a minimum; concentrations inyour PCR master mix remain consistent, leadingto stable and reproducible specificity. Nonspecificbinding is minimized beyond importance.With the Mastercycler pro system Eppendorfapplies a clear commitment to innovation withinstrument development and manufacturingexpertise to support those working in fastmoving fields. Faster speeds, highest precision,high temperature control stability, moreuser friendly, and absolute reliability united withina flexible concept: this is the definitionof the Mastercycler pro system.Product features of the Mastercycler pro‡ Ultimate reduction of evaporation‡ Extremely fast heating and cooling rates‡ Gradient blocks with SteadySlope ® Technology‡ Intuitive graphic programming‡ Display to indicate cycler number in a network‡ Upgradeable to real-time PCR‡ Control up to 5 units with one Control Panel‡ 2 year warranty‡ Optional self-test of peltier elements‡ Dual component twin.tec 1 platesprovide a stable frame andextremely thin-walled wells foroptimum heat transfer.‡ Mastercycler ep:Fast heating/cooling ratesand ultra quiet operation—in a compact design.‡ Mastercycler eprealplex: Extremelyfast optics for rapiddata acquisition.‡ Mastercycler pro:New vapo.protect technology reducesevaporation.2002 20032005 2008‡ New qPCRconsumables.Today

Eppendorf PCR consumablesSelection Guide forEppendorf MastercyclerModelCatalog No.SampleCapacityHeating/CoolingRatesMastercycler FamilyMastercycler personal* 95000003125 × 0.25 ml PCR tubes16 × 0.5 ml PCR tubesMastercycler* 9500000581 × multi-well 5 × 5 plate96 × 0.2 ml PCR tubes3 °C/s2 °C/s77 × 0.5 ml PCR tubesMastercycler gradient 950000015 1 × 96-well PCR plateMastercycler ep with motorized lidMastercycler proMastercycler ep realplexMastercycler ep gradient 950010029Mastercycler ep gradient S 95001004596 × 2 ml PCR tubes1 × 96-well PCR plateMastercycler ep gradient 384 950010061 1 × 384-well PCR plateMastercycler pro gradient 950030010Mastercycler pro gradient S 95003002096 × 2 ml PCR tubes1 × 96-well PCR plateMastercycler pro gradient 384 950030030 1 × 384-well PCR plateMastercycler ep realplex 2(2 emission filters)Mastercycler ep realplex 2 S(2 emission filters)Mastercycler ep realplex 4(4 emission filters)Mastercycler ep realplex 4 S(4 emission filters)* Model does not include gradient function.1. twin.tec plates are patented under European Patent EP 1 161 994 and US patent US 7,324,977.Eppendorf offers PCR consumables for bothstandard and real-time PCR applications. OriginalEppendorf PCR Tubes are thin-walled polypropylenetubes that ensure efficient and homogeneous heattransfer of your sample, thanks to their even wallthickness and smooth wall surface. Eppendorftwin.tec 1 PCR plates combine a polycarbonate frameand polypropylene wells for optimum performance,and our real-time PCR plates offer the advantageof white wells that reflect fluorescence better thanclear or frosted wells.Learn more about these products and limited-timesavings offers at: www.eppendorfna.com/mastercyclerPleaseinquire96 × 0.2 ml PCR tubes1 × 96-well PCR plate4 °C/s3 °C/s6 °C/s4.5 °C/s4 °C/s3 °C/s4 °C/s3 °C/s6 °C/s4.5 °C/s4 °C/s3 °C/sBlockAluminumAluminumSilverAluminumAluminumSilverAluminum4 °C/s3 °C/sAluminum6 °C/s4.5 °C/sSilver4 °C/s3 °C/sAluminum6 °C/s4.5 °C/sSilverAll models are 120 V/50–60 Hz.PCR.A1.0101.A.US-2008.0 © 2008 Eppendorf AG. Eppendorf ® , Mastercycler ® , vapo.protect and SteadySlope ® are registered trademarks of Eppendorf AG. Practice of the patented polymerase chain reaction (PCR)process requires a license. The Mastercycler is an Authorized Thermal Cycler and may be used with PCR licenses available from Applied Biosystems. Its use with Authorized Reagents also provides a limited PCR licensein accordance with the label rights accompanying such reagents. All rights reserved, including graphics and images. Product appearances, specifications, and/or prices are subject to change without notice.www.eppendorf.com • Email: info@eppendorf.com • Application hotline: 516-515-2258In the U.S.: Eppendorf North America, Inc. 800-645-3050 • In Canada: Eppendorf Canada Ltd. 800-263-8715