TOXICOLOGICAL PROFILE FOR CHROMIUM - Davidborowski.com

Sample matrixTable 6-1. Analytical Methods for Determining Chromium in Biological MaterialsPreparation methodPlasma Wet ashing with HNO 3 /HCIO 4 /H 2 SO 4 ;residue complexed with APDC andextracted with MIBK; evaporatedresidue dissolved deposited inHNO 3 /HCE, and solution on apolycarbonate foilBlood, serumSerumSample after wet digestion convertedto a volatile chelate usually withfluorinated acetylacetoneMg(NO 3 ) 3 added to serum, dried byLyophilization, ashed, and dissolvedin 0.1 N HCIBlood Diluted with 0.1% EDTA and 5%isopropanolAnalyticalmethodSampledetection limitPercentrecoveryReferencePIXE 0.3 µg/L 87% at 4.5 µg/g Simonoff et al. 1984GC/ECD0.03 pg0.5 pg1.0 ngNo data Fishbein 1984GFAAS 0.005 µg/L 103% at 0.30 µg/L Randall and Gibson 1987GFAAS-ZeemaneffectbackgroundcorrectionBlood or tissue Wet ashing with HNO 3 /HCIO 4 /H 2 SO 4 ICP-AES 1 µg/100 g blood0.2 µg/g tissue0.09 µg/L No data Dube 1988114% recovery at10 µg/sampleNIOSH 1994a(Method No. 8005)Erythrocytes Dilution with Triton X100 GFAAS No data No data Lewalter et al. 1985Serum and urine HNO 3 de-proteinization GFAAS withpyrolytic graphitetube and ZeemanbackgroundcorrectionBody fluids (milk,urine, etc.)Dried sample ashed by oxygenplasma, H 2 O 2 addition, drying,dilution in 1N HClGFAAS withtungsten iodide ordeuterium arc orCEWMbackgroundcorrection0.02 µg/L (serum)0.1 µg/L (urine)No data Sunderman et al. 1989