Curriculum vitae

Curriculum vitaeName: Hilde-Gunn Opsahl Sorteberg (Ferstad until Aug.2003), Born 4 th April 1963, Norwegian.E.mail: hildop@umb.noEducation & Positions:2004-2005 Visiting scholar UC-Berkeley/USDA, PGEC, Jenn Fletchers group.2004- Professor in Molecular Biology/Genetics IPM, UMB2000-2004 Group leader Lab for Molecular Plant Biology (LMPB) at IKB, AUN with 12 members1998- Associate professor in plant molecular genetics, IKB, AUN1996-99 Projectleader ‘Transformation of cereals’ (Molecular Genetics)Rice, Wheat and BarleyEndosperm development1994-96 Post doc RCAP/INRA (Molecular Genetics)ENS-Lyon, FranceEmbryogenesis, Maize1994 Post doc.Dept of Biotechnological Sciences (IBF)Population Genetics1993 Post doc.Dept Biology & ConservationPlant Physiology1993 PhD (Dr scient) Genetics & Plant BreedingAUNMolecular BiologySelected administrative experiences and other important exercises:Invited member of ERA-NET initiative, EPSO and EU project and proposal meetings.Stand in member of the University board (AUN) 2001-2005Board member Dept. of Chemistry & Biotechnology 2002-2003Head of Section Genetics 2002-2004Leader of the employees sports association AUN 2001-2003Member and following leader of the teaching board at the Dept. 1999-2003Boardmember Dept. of Biotechnology 1997-1998Member of the University advisory board 1990-92Student member of the board of Dept of Genetics and Plant Breeding 1986/87Leader of IAESTE (Int. Ass. Exchange Students Techn. Exp.)3 yearsJournalist in the student paper ‘Tuntreet’3 yearsCorreografer & dancer revue cavalcade 1989, AUN1 monthDancer UKA-86, AUN2.5 monthsActor UKA-84, AUN2.5 monthsNorwegian research stations; Stjørdalen-85 and Tromsø-84, total4 monthsIAEA exchange student Australia 1983-846 monthsNorwegian Research Station Tjøtta3 monthsEditor of the student graduation newspaper 1981-2Teaching experience:1988- GN200, BIO120, BIO220, BIO310, BIO311, BIO322, GN310 Genetics/Mol.Biol.1999- Supervised 20 master students, and 2 PhD students (Ronald Al, Lene T Olsen)2000- “Sensor” final examinations coarses and theses for Cand. Scient UiO 10 candidates2001, 2002 Oponent on PhD defences at AUN and UiO (Morten Lillemo, May Kristin Lyamouri)Member PhD committees I Mercy, SE Lid and Cheng Fang2003 Responsible for NOVA coarse in BioethicsOverview of main projects:*NFR NKJ project (2005-2006) Functional Genomics of Starch and Grain Quality: A Nordicmultidisciplinary program*NFR project 2004-2007 “Functional studies of key regulatory genes in cell fate specification ofaleurone cells with application to cereal breeding”, NFR-159031/120 (just under 2 mill per year)*NFR project 2003 (154644/130)-Adapting the fatty acid composition of rapeseed to meet future feedneeds for farmed fish*NFR project 2000-2003 (133846/130) Isolering av gener for endospermutvikling til bruk ikornforedling, 1.2 mill pr år

Patent references:-Ltp2 promoter having aleurone-tissue specific activity. United States Patent 5,525,716.Promoter” (Ltp2 promoter having aleurone-tissue specific activity). UK patent No 9611388.1This promoter is currently being evaluated and tested at Pioneer Hi-Bred/DuPont and Zenneca.-Transgenic organism PCT patent application No.PCT/ NO95700042. The use of two barley promotersfor expression of GOIs in the grains of transgenic plants. The Ltp1 and B22E promoters-UKPatent Transgenic organism (The use of AGPase cDNAs from barley endosperm to enhancestarch production in transgenic plants). (UK Patent Application 9520520.9).This patent is owned by Danisco A/S (DK), and covers two barley genes.-ESR genes isolated in France patented by Biogemma (Opsahl-Ferstad et al. 1997)-AGPase to increase β-glucan and reduce starch in cereals filed 2004 and 2006Invited speaker/presentations at international meetings: national meetings 2002/3:UC-Berkeley, USA 2004, 2005Gordon Conference, USA 2004Plant and Animal Genome XI,NBS vintermøtet RørosSan Diego, USA January 2003 Rotary Holmestrand (11 mars)Apomixis meeting Como,I, April 2001Nettverket for trygg mat 13. aprilJohn Innes Centre, UK, 2001 Utviklingsfondet 25. aprilRDP, ENS-Lyon, F 1999, 2000 GMO fagdag MATFORSKRisø Research Centre, DK 1999 NITO (Folkehelsa, DYNAL) 31. OktNBS biochemical soc. 1997,-99, 2000, 2001 Globaliseringskonf. Oslo 2 nov. 2003Collaborations:Pioneer Hi-Bred/DuPont collaboration secured by several contracts, exchange of students and labor,joint patenting and publications.JIC, John Innes Centre, UK, on transgenic technology visiting scientist in Paul Christou’s lab 1-2months per year 1996-2001, Wendy Harwood/Philippe Vaugh/John Snape 2001-, on starch synthesisAlison Smith & Kay Denyer.Peter Sandøe (Centre for Bioethics & Risk Assessment/CeBRA) PhD coarses + projectproposals.Timothy Close (UC Riverside, USA) on Affymetrix transcription profiling from summer 2003.Aquaculture Protein Centre (APC/CoE)/Akvaforsk (Ståle Refstie & Bente Reyter) on evaluating thepotential use of oil from Rapeseed/Canola to feed fish using microarray transcript profiling,transgenics, cell cultures and feed experimentation initiated 2003.Genetwister Technologies B.V. (Wageningen, NL) on functional genomics of putative Arabidopsisligand knoc-outs, with Ed Schmidt supervising master student Ingrid Roxrud thesis work with them.Alan H. Schulman University of Helsinki, Inst. of Biotechnology & Plant Breeding Biotechnology, MTTAgrifood Research Finland. NKJ project collaboration. Christer Jansson Uppsala, S, & AndreasBlennow, Lars Munck and Søren Rasmussen KVL, DK.Jennifer C Fletcher UC-Berkeley (USA) on the GASA genes involvement in flower development.NARC-FUGE platform placed in our lab.Fred Berger previously at RCAP Lyon, now in Singapore.

Scientific Publications:Lene T. Olsen, Ronald H. Al, Stein Erik Lid, Runar Andreassen, Kjetil Fosnes andHilde-Gunn Opsahl-Sorteberg (Work in prep.) Characterization of the barley B5 aleuronemutant and expression analysis of selected endosperm developmental genes in wildtypebarley.Ingrid Roxrud, Stein Erik Lid, Jennifer C Fletcher, Ed DL Schmidt and Hilde-Gunn Opsahl-Sorteberg *(Submitted) GASA4, one of the 14 member Arabidopsis GASA family of small polypeptides, isrequired for flower and seed development. Plant Cell & PhysRosti S, Rudi H, Rudi K, Opsahl-Sorteberg HG, Fahy B and Denyer K (2006) The gene encoding thecytosolic small subunit of ADP-glucose pyrophosphorylase in barley endosperm also encodesthe major plastidial small subunit in the leaves. J Exp Bot [Epub ahead of print]Lid SE, Olsen L, Nestestog R, Aukerman M, Brown RC, Lemmon B, Mucha M, Opsahl-Sorteberg HG,Olsen OA. (2005) Mutation in the Arabidopisis thaliana DEK1 calpain gene perturbsendosperm and embryo development while over-expression affects organ developmentglobally. Planta, 221:339-351.Opsahl-Sorteberg * H-G, Rudi H, Lid SE and Schulman AH (2005) Understanding developmentalbiology opens vast opportunities for designing novel feed and food – a discussion of potentialnew plant products. IN: “In the wake of the double helix” In press (pdf:200kB)Rudi H, Frisli T & Opsahl-Sorteberg H-G (2004) Hordeum vulgare gene for ADP-glucosepyrophosphorylase small subunit, complete sequence Accession no AY634681Opsahl-Sorteberg H-G*, Nielsen PS, Lund HH, Kalla R, Hammond-Kosack MCU, Shimamoto K(2004) Identification of a 49 bp fragment of the HvLTP2 promoter directing aleurone cellspecific expression. GENE (27)341:49-58Opsahl-Sorteberg H-G*, Mercy IS, Kolderup E, Ramstad VE, Linnestad C, Harwood WA andShimamoto K (2004) A study of cell specific expressiondirected by the barley LTP1(HvLTP1) promoter in barley and rice. In press 124-BiotechnologyStein Erik Lid, Ronald Al, Trygve Krekling, Robert B. Meeley, Jerry Ranch, Hilde-Gunn Opsahl-Ferstadand Odd-Arne Olsen (2004) The maize disorganized aleurone layer 1 and 2 (dil1, dil2)mutants lack control of mitotic division plane in the aleurone layer of developing endosperm.PLANTA 218:370-8.Opsahl-Ferstad H-G, Rudi H, Ruyter B and Refstie S (2003) Biotechnological approaches to modifyrapeseed oil composition for applications in aquaculture. Plant Science 165: 349-357Jansson, C and Opsahl-Ferstad, H-G (2003) Transgenics and mutants - a comparison of barleyresources. Progress in Botany 64:42-52Opsahl-Ferstad H-G (2002) New intelligent tools to understand seed development and possibleimplications to molecular farming (Invited review) PJAS 2(1):39-43Becraft, P.W., Brown, R.C., Lemmon, B.E., Olsen, O.-A., and Opsahl-Ferstad, H.-G. (2001)Endosperm development. In: S.S. Bhojwani and W.Y. Soh (eds) Current Trends in theEmbryology of Angiosperms. Kluwer Academic Publ., Dordrecht, pp. 353-374.Opsahl-Ferstad H-G and Rudi H (2000) Endosperm Development, Gene Regulation and StarchSynthesis in Cereals. Current Topics in Plant Biology 2:83-92Thorbjørnsen T, Villand P, Ramstad VE, Rudi H, Kleczkowski LA, Olsen O-Aand Opsahl-Ferstad H-G* (2000) Nucleotide sequence of the ADP-glucosepyrophosphorylase promoter (Accession No. AJ239130) of barley (Hordeum vulgare L.), amajor controlling gene in endosperm starch synthesis. Plant Phys. 122: 1457Bonello J-F, Opsahl-Ferstad H-G, Perez P, Dumas C, Rogowsky P (2000) Esr genes show differentlevels of expression in the same region of maize endosperm. GENE 246:219-227Opsahl-Ferstad H-G, Le Deunff E, Dumas C & Rogowsky P (1997) ZmEsr, a novelendosperm specific gene expressed in a restricted region around the maize embryo,The Plant Journal 12(1):235-246Opsahl-Ferstad H-G, Bjornstad A, Rognli OA (1994) Genetic control of androgenesis inLolium perenne L., Theor Appl Genet 89:133-138Opsahl-Ferstad H-G, Bjornstad A, Rognli OA (1994) Influence of pretreatment and mediumof androgenetic response in Lolium perenne L., Pl Cell Reports 13:594-600Aalen RB, Opsahl-Ferstad H-G, Linnestad C, Olsen O-A (1994) Transcripts encoding anoleosin and a dormancy-related protein are present in both the aleurone layer and theembryo of developing barley (Hordeum vulgare L.) seeds, The Plant Journal 5:385-396Bjornstad A, Opsahl-Ferstad H-G, Aasmo M (1989) Effects of donorplant environmentand light during incubation on anther cultures of some spring wheat(Triticum aestivum L.) cultivares, Pl Cell Tiss Org Cult (17):27-37

Berg T, Syed M, Opsahl-Ferstad H-G, Tsegaye B and Waktola A (2003) Biotechnology in developingcountries: Need and modes of competence building. A Noragric Study for Norad. 33 p.