ab150681 Picro-Sirius Red Stain Kit (Connective Tissue ... - Abcam

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1. IntroductionThe The Picro-Sirius Red Stain Kit (Connective Tissue Stain) isintended for use in the histological visualization of collagen I and IIIfibers in addition to muscle in tissue sections. The PSR stain maybe viewed using standard light microscopy or polarized light resultingin birefringence of the collagen fibers to distinguish between type Iand type III.Staining Interpretation:Light MicroscopyCollagenMuscle FibersCytoplasmPolarized Light MicroscopyType I (Thick fibers)Type III (Thin fibers)RedYellowYellowYellow/Orange BirefringenceGreen BirefringenceControl Tissue:Lung, Uterus, Muscle, Kidney.3
2. Kit ContentsComponents Amount StoragePicro-Sirius Red Solution 250 ml RTAcetic Acid Solution (0.5%) 2 x 250 ml RT3. Storage and HandlingFor storage temperatures please see the TableKeep away from open flame. Refer to the Safety Data Sheet.4. Additional Materials RequiredAbsolute alcohol.Distilled water.4
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ab150681 Picro-Sirius Red Stain Kit (Connective Tissue ... - Abcam

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