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Research concerning the composition and organoleptic features

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Studies on <strong>the</strong> influence of genotype <strong>and</strong> culture media<strong>composition</strong> on growing explants in <strong>the</strong> in vitro initiation phaseTudor Radu C.M. 1* , Radomir Ana Maria 11 National <strong>Research</strong> & Development Institute for Biotechnology in Horticulture Stefanesti, Sos. Bucuresti-Pitesti, no. 37, CP 117713, Arges, ROMANIA*Corresponding author. Email: mihai_radutudor@yahoo.co.ukAbstract To initiate ‘in vitro’ cultures <strong>the</strong>re were sampled shoots fromroses grown in soil <strong>and</strong> in <strong>the</strong> greenhouse <strong>and</strong> from blackberry grown in pots.The used roses genotypes were Thea hybrid (Akito, Crimson Glory,Baccara) <strong>and</strong> climbing roses (New Dawn <strong>and</strong> Ilse Krohn Superior).The meristemes used for ‘in vitro’ culture initiation were sampledunder <strong>the</strong> magnifying glass in laminar air flow.For <strong>the</strong> meristemes growing it was assured a 22-24 0 C temperature in<strong>the</strong> room <strong>and</strong> 16 hours of light with 2500 lux intensity.Nutrient mediums used for <strong>the</strong> initiation phase of <strong>the</strong> cultures weredifferent in <strong>composition</strong>.The rose varieties have performed well in <strong>the</strong> growth of explants, <strong>the</strong>differences being <strong>the</strong> result of interaction of genotype with <strong>the</strong> culture mediumcomponents.Regarding <strong>the</strong> influence of culture medium, as abiotic factor, it was found that<strong>the</strong> highest values were obtained for explants grown on culture mediumMurashige - Skoog, with 0,004 mg / l naftilacetic acid / 2 mg / lbenzilaminopurine. Decreased concentration of cytokinine led to lowerpercentage of grown explants.The growing of blackberry explants was influenced by <strong>the</strong> genotype <strong>and</strong> by<strong>the</strong> culture medium components.Key words‘in vitro’ culture, culturemedium, Thea hybrids<strong>Research</strong> <strong>concerning</strong> <strong>the</strong> content of cations <strong>and</strong> anions in <strong>the</strong>groundwater of <strong>the</strong> Timis riverAntoanela Cozma 1 , A.Lazureanu 2 , Alina Balint 2 , Alexa Ersilia 31 Banat‟s University of Agricultural Sciences <strong>and</strong> Veterinary Medicine, Department of Exact Siences,Timisoara, Aradului Street no.119, RO-300645, Romania2 Faculty of Horticulture <strong>and</strong> Forestry, USAMVB Timisoara, Faculty of Agro-Food Processing TechnologiesTimisoara*Corresponding author. Email: antoanelacozma@yahoo.comAbstract In this study <strong>the</strong> objectives is <strong>the</strong> monitorizing of <strong>the</strong> cations(iron <strong>and</strong> manganese) <strong>and</strong> anions (sulfates, phosphates, chlorides) content ofgroundwater in <strong>the</strong> five prelevation places situated on <strong>the</strong> Timis river way,during <strong>the</strong> 2004 year. The obtained results were compared to <strong>the</strong> limit valuesof <strong>the</strong> chemical indicators established in actual legislation. The obtainedvalues showed exceeding of <strong>the</strong> maximum admitted limits at points locateddownstream from pollution sources.The poluted sources are caused bydischarge wastewater stations appeared in <strong>the</strong> sewage channel of Lugoj city<strong>and</strong> also from Bega-Timis discharge channel.Key wordsanions, cations content,chemical parameters,pollution sources

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