Persicaria odorata Antibacterial Activity of the ... - ResearchGate

Persicaria odorata Antibacterial Activity of the ... - ResearchGate Persicaria odorata Antibacterial Activity of the ... - ResearchGate

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106 108ปี ที 42 ฉบับที 2 (พิเศษ) พฤษภาคม - สิงหาคม 2554 ว. วิทยาศาสตร์เกษตรConner, D.E., 1993, Naturally Occurring Compounds, pp. 441-468. In P.M. Davidson and A.L. Branen (eds.).Antimicrobials in Foods. 2nd ed. Marcel Dekker, Inc., New York.Hashemi, P., Abolghasemi, M.M., Ghiasvand, A.R., Ahmadi, S., Hassanvand, H. and Yarahmadi, A., 2008, AComparative Study of Hydrodistillation and Hydrodistillation–Solvent Microextraction Methods forIdentification of Volatile Components of Echinophora cinerea, Journal of Chromatographia, 69: 179-182.Matasyoh, J.C., Maiyo, Z.C., Ngure, R.M. and Chepkorir, R., 2008, Chemical Composition and AntimicrobialActivity of The Essential Oil of Coriandrum sativum, Journal of Food Chemistry, 113(2): 526-529.Laohakunjit, N., * Orapin, K., Krittika, N. and Pantip, B, 2008, Essential Oil from Five Zingiberaceae for AntiFood-Borne Bacteria, International Food Research Journal, 15(3): 337-346.Singhal, R.S., Kulkarni, P.R. and Rege, D.V., 2001, Quality Indices for Spice Essential Oils, Chapter 3 pp. 30.InPeter, K.V., Handbook of Herbs and Spices, CRC Press, USA.Sadashiva, C.T., Sharanappa, P., Remashree, A.B., Raghu, A.V., Udayan, P.S. and Balachandran, I., 2010,Chemical Composition and Antimicrobial Activity of the Essential Oil from Bark of Pittosporum dasycaulonMiq., Advances in Biological Research, 4(6): 301-304.Yentema, O., Alioune, O. and Dorosso, S.A., 2007, Chemical Composition and Physical Characteristic of theEssential oil of Cymbopogon schoenanthus (L.) Spreng of Burkina Faso, Journal of Applied Sciences,7(4): 503-506.Table 1 Physical properties of essential oil from fresh and dry leaves of Persicaria odorataSample Yield of extraction L* a* b* c* Hue angle Color appearance Refractive index(%)-dry basisFresh 0.274 70.61 -0.86 2.50 2.64 108.99 Light green 1.472Dry 0.120 69.96 -0.86 2.26 2.42 110.83 Light yellow 1.471Table 2 Constituent of the essential oil from fresh and dry leaves of Persicaria odorataNo. Volatile compounds RI a Composition (%)No. Volatile compounds RI a Composition (%)Fresh Dry Fresh Dry1. eucalyptol 1051 0.31 - 14. alpha,-curcumene 1490 3.16 4.492. undecane 1108 - 0.14 15. eremophillene 1508 6.89 4.203. 1-nonanol 1174 - 0.09 16. 7-epi-alpha-selinene 1537 - 2.594. decanal 1212 7.32 4.47 17. ledol 1550 5.99 -5. decanol 1274 - 3.34 18. nerolidol 1564 - 3.676. undecanal 1311 0.58 0.57 19. globulol 1587 0.95 -7. n-decanoic acid 1358 0.21 - 20. caryophyllene oxide 1601 2.04 5.648 1-nonene 1374 - 2.02 21. cubenol 1640 0.08 -9. beta-elemene 1401 0.64 - 22. eupatoriochromene 1664 21.71 20.9410. dodecanal 1417 19.96 18.72 23. drimenol 1790 4.74 4.3411. beta-caryophyllene 1441 11.07 11.40 24. hexahydro farnesyl acetone 1842 - 0.6012.13.allo-aromadendrenalpha.-caryophyllene145614760.4912.57-11.6225.26.isophytoln-hexadecanoic acid194519590.390.910.170.99a Retention indices calculated based on n-alkane standard (C 10 -C 20 )Table 3 Antibacterial activity and Minimum inhibitory concentration (MIC) value of essential oil from fresh anddry leaves of Persicaria odorataBacterial speciesInhibition zones (mm)*MIC value(µl/ml)Fresh Dry DMSO** Streptomycin (5mg/ml)*** Fresh DryS. aureus 21 26 - 23 6.25 6.25E. coli 13 19 - 20 12.5 12.5*Inhibition zone including the diameter of the paper disc (6 mm); ** Negative control (DMSO); *** Positive control (5mg/ml Streptomycin)

106 108ปี ที 42 ฉบับที 2 (พิเศษ) พฤษภาคม - สิงหาคม 2554 ว. วิทยาศาสตร์เกษตรConner, D.E., 1993, Naturally Occurring Compounds, pp. 441-468. In P.M. Davidson and A.L. Branen (eds.).Antimicrobials in Foods. 2nd ed. Marcel Dekker, Inc., New York.Hashemi, P., Abolghasemi, M.M., Ghiasvand, A.R., Ahmadi, S., Hassanvand, H. and Yarahmadi, A., 2008, AComparative Study <strong>of</strong> Hydrodistillation and Hydrodistillation–Solvent Microextraction Methods forIdentification <strong>of</strong> Volatile Components <strong>of</strong> Echinophora cinerea, Journal <strong>of</strong> Chromatographia, 69: 179-182.Matasyoh, J.C., Maiyo, Z.C., Ngure, R.M. and Chepkorir, R., 2008, Chemical Composition and Antimicrobial<strong>Activity</strong> <strong>of</strong> The Essential Oil <strong>of</strong> Coriandrum sativum, Journal <strong>of</strong> Food Chemistry, 113(2): 526-529.Laohakunjit, N., * Orapin, K., Krittika, N. and Pantip, B, 2008, Essential Oil from Five Zingiberaceae for AntiFood-Borne Bacteria, International Food Research Journal, 15(3): 337-346.Singhal, R.S., Kulkarni, P.R. and Rege, D.V., 2001, Quality Indices for Spice Essential Oils, Chapter 3 pp. 30.InPeter, K.V., Handbook <strong>of</strong> Herbs and Spices, CRC Press, USA.Sadashiva, C.T., Sharanappa, P., Remashree, A.B., Raghu, A.V., Udayan, P.S. and Balachandran, I., 2010,Chemical Composition and Antimicrobial <strong>Activity</strong> <strong>of</strong> <strong>the</strong> Essential Oil from Bark <strong>of</strong> Pittosporum dasycaulonMiq., Advances in Biological Research, 4(6): 301-304.Yentema, O., Alioune, O. and Dorosso, S.A., 2007, Chemical Composition and Physical Characteristic <strong>of</strong> <strong>the</strong>Essential oil <strong>of</strong> Cymbopogon schoenanthus (L.) Spreng <strong>of</strong> Burkina Faso, Journal <strong>of</strong> Applied Sciences,7(4): 503-506.Table 1 Physical properties <strong>of</strong> essential oil from fresh and dry leaves <strong>of</strong> <strong>Persicaria</strong> <strong>odorata</strong>Sample Yield <strong>of</strong> extraction L* a* b* c* Hue angle Color appearance Refractive index(%)-dry basisFresh 0.274 70.61 -0.86 2.50 2.64 108.99 Light green 1.472Dry 0.120 69.96 -0.86 2.26 2.42 110.83 Light yellow 1.471Table 2 Constituent <strong>of</strong> <strong>the</strong> essential oil from fresh and dry leaves <strong>of</strong> <strong>Persicaria</strong> <strong>odorata</strong>No. Volatile compounds RI a Composition (%)No. Volatile compounds RI a Composition (%)Fresh Dry Fresh Dry1. eucalyptol 1051 0.31 - 14. alpha,-curcumene 1490 3.16 4.492. undecane 1108 - 0.14 15. eremophillene 1508 6.89 4.203. 1-nonanol 1174 - 0.09 16. 7-epi-alpha-selinene 1537 - 2.594. decanal 1212 7.32 4.47 17. ledol 1550 5.99 -5. decanol 1274 - 3.34 18. nerolidol 1564 - 3.676. undecanal 1311 0.58 0.57 19. globulol 1587 0.95 -7. n-decanoic acid 1358 0.21 - 20. caryophyllene oxide 1601 2.04 5.648 1-nonene 1374 - 2.02 21. cubenol 1640 0.08 -9. beta-elemene 1401 0.64 - 22. eupatoriochromene 1664 21.71 20.9410. dodecanal 1417 19.96 18.72 23. drimenol 1790 4.74 4.3411. beta-caryophyllene 1441 11.07 11.40 24. hexahydro farnesyl acetone 1842 - 0.6012.13.allo-aromadendrenalpha.-caryophyllene145614760.4912.57-11.6225.26.isophytoln-hexadecanoic acid194519590.390.910.170.99a Retention indices calculated based on n-alkane standard (C 10 -C 20 )Table 3 <strong>Antibacterial</strong> activity and Minimum inhibitory concentration (MIC) value <strong>of</strong> essential oil from fresh anddry leaves <strong>of</strong> <strong>Persicaria</strong> <strong>odorata</strong>Bacterial speciesInhibition zones (mm)*MIC value(µl/ml)Fresh Dry DMSO** Streptomycin (5mg/ml)*** Fresh DryS. aureus 21 26 - 23 6.25 6.25E. coli 13 19 - 20 12.5 12.5*Inhibition zone including <strong>the</strong> diameter <strong>of</strong> <strong>the</strong> paper disc (6 mm); ** Negative control (DMSO); *** Positive control (5mg/ml Streptomycin)

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