Visual Psychophysics / Physiological Optics - ARVO

ARVO 2013 Annual Meeting Abstracts by Scientific Section/Group – Visual Psychophysics / Physiological OpticsClinical Trial: C10-03Program Number: 6059 Poster Board Number: B0070Presentation Time: 10:30 AM - 12:15 PMInvestigation of the correlation among diameter of retinal vesseland arteriosclerosis risk factors including hypoadiponectinemiaKei Homma 1 , Ryo Kawasaki 2 , Yusuke Tanabe 1 , Koko Saito 1 , AkiraSugano 1 , Atsushi Hozawa 3 , Katou Takeo 4 , Isao Kubota 5 , TakamasaKayama 6 , Hidetoshi Yamashita 1 . 1 Ophthalmology, Yamagata UnivSch of Medicine, Yamagata, Japan; 2 Public Health, Yamagata UnivSch of Medicine, Yamagata, Japan; 3 Tohoku Univ Sch of Medicine,Sendai, Japan; 4 Neurology,Hematology,Metabolism,Endocrinologyand Diabetology, Yamagata Univ Sch of Medicine, Yamagata, Japan;5 Cardiology,Pulmonology,and Nephrology, Yamagata Univ Sch ofMedicine, Sendai, Japan; 6 Neurosurgery, Yamagata Univ Sch ofMedicine, Yamagata, Japan.Purpose: Hypoadiponectinemia caused by visceral fat accumulationis related to hypertension, diabetes, and hyperlipidemia, and is anindependent risk factor for the arteriosclerosis.In this research, the correlation among diameter of retinal vessel andarteriosclerosis risk factors including hypoadiponectinemia wasinvestigated in the population based epidemiological study.Methods: This research was approved by the Ethics committee ofYamgatata University School of Medicine. Fundus photographs weretaken in the subjects who participated in the Takahata study andanalyzed after securing written permission from the subjects. Intotal,1473 subjects were investigated.The estimated retinal artery(CRAE:centaral retinal artery equivalent,the estimated retinal vein(CRVE:central retinal vein equivalent),andAVR (arteliole to venule ratio) were measured from the fundusphotographs used by the vessel diameter measurement dedicatedsoftware (Retinal Analysis,University of Wisconsin,Wisconsin).Characteristics to be inspected were divided into about 4groups, and the vessel diameter of an each part was analyzed.Results: Multiple linear regression analysis showed that CRAEdecreased along with systolic arterial pressure increased(-0.067μm;p=0.039).CRAE increased along with adiponectin levelincreased(0.153μm;p=0.049).CRVE increase was also associatedwith HbA1c,smoking index,and body massindex(3.333μm;p=0.004,0.004μm;p=0.023,0.412μm;p=0.007respectively).Conclusions: Hypoadiponectinemia was an independent risk factorfor CRAE decrease.The results suggest that measurement of retinalblood vessels are a good clinical marker to detect macrovasculardiseases.Commercial Relationships: Kei Homma, None; Ryo Kawasaki,None; Yusuke Tanabe, None; Koko Saito, None; Akira Sugano,None; Atsushi Hozawa, None; Katou Takeo, None; Isao Kubota,None; Takamasa Kayama, None; Hidetoshi Yamashita, Senju (C),Senju (P)Program Number: 6060 Poster Board Number: B0071Presentation Time: 10:30 AM - 12:15 PMNoninvasive Imaging of Retinal Pericytes and Endothelial Cellsin Living Human EyesToco Y. Chui, Thomas Gast, Stephen A. Burns. Optometry, IndianaUniversity, Bloomington, IN.Purpose: To image the retinal vascular pericytes and endothelialcells in living human retina using an adaptive optics scanning laserophthalmoscope (AOSLO).Methods: In vivo arteriolar wall imaging was performed on 8 healthysubjects using the Indiana AOSLO (Ferguson et al, 2010). Vesselwall imaging was performed using systematic control of the positionof a large confocal aperture (Chui et al, 2012). Peripapillary arteriesand arterioles were divided into 4 groups based on their lumendiameters (Gr 1: ≥100µm; Gr 2: 50-99µm; Gr 3: 10-49µm; Gr 4:retinal capillaries).Results: The retinal microvasculature and scattering behavior oferythrocytes were clearly visualized in all 8 subjects. On the smallervessels the pericytes were visualized as distinct cells laying along thelumen of the blood vessel. The smaller vessel pericytes bulgedoutward from the vessel wall resulting in a wall of irregularthickness. For the larger Gr 1 vessels the pericytes were much flatterand formed the outer corrugated layer of a two (or more) layeredvascular wall. While pericytes were readily seen in vessel groups 1,2, and 3, endothelial cells were only visible in Gr 1 vessels - thelargest retinal arteries. Fig 1A shows the fine structure of arteriolarwall in a Gr 1 and Gr 3 vessel. The ratio of pericytes to endothelialcells was approximately 1:1 in the Gr 1 arteries (Fig 1B). Vascularwall components were not identified in the smallest retinal capillaries(Gr 4).Conclusions: Our results show that retinal pericytes can be readilyresolved in normal subjects for arterioles with a lumen diameter>10µm. Our noninvasive imaging approach allows direct assessmentof the cellular structure of the vascular wall in vivo with potentialapplications in retinal vasculopathies such as diabetic retinopathy.Fig1 A) Fine structure of vessel wall in peripapillary arterioles.Asterisks indicate the vessel lumen of a 100µm diameter and 14µmdiameter arteriole. The inner and outer vessel wall linings of thelarger arteriole are indicated by the white arrows. Pericytes (blackarrows) are observed in a precapillary arteriole with a lumen diameterof 14µm. B) Magnified region of the white box on A. Endothelialcells (white arrows) and pericytes (black arrows) are readily seenalong the inner and outer vessel wall linings. Note the one to oneratio of endothelial cells and pericytes.Commercial Relationships: Toco Y. Chui, None; Thomas Gast,None; Stephen A. Burns, NoneSupport: NIH grants R01-EY14375, R01-EY04395, andP30EY019008Program Number: 6061 Poster Board Number: B0072Presentation Time: 10:30 AM - 12:15 PM©2013, Copyright by the Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Go to iovs.org to access the version of record. For permissionto reproduce any abstract, contact the ARVO Office at arvo@arvo.org.