Spectrophotometric determination of carbamazepine ... - MA Zayed
Spectrophotometric determination of carbamazepine ... - MA Zayed Spectrophotometric determination of carbamazepine ... - MA Zayed
Spectrophotometric determination of carbamazepine and mosapride citrate in pure and in pharmaceutical preparations. Eman Y.Z. Frag, M.A. Zayed*, M.M. Omar, Sally E.A. Elashery, Gehad G. Mohamed Chemistry Department, Faculty of Science, Cairo University, Gamaa Str., 12613, Giza, Egypt * Author for whom all correspondence should be addressed. (e-mail: mazayed429@yahoo.com) 1
Spectrophotometric determination of carbamazepine and mosapride citrate in pure and pharmaceutical preparations. Eman Y.Z. Frag, M.A. Zayed*, M.M. Omar, Sally E.A. Elashery, Gehad G. Mohamed Chemistry Department, Faculty of Science, Cairo University, Gamaa Str., 12613, Giza, Egypt * Author for whom all correspondence should be addressed. (e-mail: mazayed429@yahoo.com) Abstract Simple, rapid and sensitive spectrophotometric methods were developed for the determination of carbamazepine and mosapride citrate drugs in pure and pharmaceutical dosage forms. These methods are based on ion pair and charge transfer complexation reactions. The first method is based on the reaction of the carbamazepine drug with Mo(V)-thiocyanate in hydrochloric acid medium followed by extraction of the coloured ion-pair with 1,2-dichloroethane and measured at 470 nm. The second method is based on the formation of ion-pairs between mosapride citrate and two dyestuff reagents namely bromothymol blue (BTB) and bromocresol green (BCG) in universal buffer of pH 4 and 3, respectively. The formed ion-pairs are extracted with chloroform and methylene chloride and measured at 412 and 416 nm for BTB and BCG reagents, respectively. The third method is based on charge transfer complex formation between mosapride citrate (electron donor) and DDQ (π-acceptor reagent) and measurements at 450 nm. All the optimum conditions are established. The calibration graphs are rectilinear in the concentration ranges 10-350 for carbamazepine using Mo(V)-thiocyanate and 4-100, 4-60 and 10-150 μg mL -1 for mosapride citrate using BTB, BCG and DDQ reagents, respectively. The Sandell sensitivity (S), molar absorptivity, correlation coefficient, regression equations and limits of detection (LOD) and quantification (LOQ) are calculated. The law values of standard deviation (0.04-0.09 for carbamazepine using Mo(V)-thiocyanate and 0.022-0.024, 0.013-0.018 and 0.013-0.020 for mosapride citrate using BTB, BCG and DDQ, respectively) and relative standard deviation (0.630-2.170 for carbamazepine using Mo(V)-thiocyanate and 0.123-1.43, 0.102-0.530 and 0.226-1.280 for mosapride citrate using BTB, BCG and DDQ, respectively) reflect the accuracy and precision of the proposed methods. The methods are applied for the assay of the investigated two drugs in pharmaceutical dosage forms. The results are in good agreement with those obtained by the official method. Keywords: Carbamazepine and mosapride citrate; Ion-pair formation; Charge transfer; Spectrophotometry. 2
- Page 1: Accepted Manuscript Original articl
- Page 5 and 6: Cl H 2 N O HO HO O O O OH H N O OH
- Page 7 and 8: lank solution. Procedure for the ta
- Page 9 and 10: carbamazepine. The data obtained ar
- Page 11 and 12: Cl + HO - H 2 N O Br HO HO O O O OH
- Page 13 and 14: Cl Cl Cl O O DDQ CN CN + H 2 N O HO
- Page 15 and 16: Mohamed, G.G., Nour El-Dien, F.A.,
- Page 17 and 18: Table (2). The molar absorptivity v
- Page 19 and 20: Table (4). Spectrophotometric deter
- Page 21 and 22: Absorbance 0.4 0.3 0.2 0.1 0 0 20 4
- Page 23 and 24: a b Figure (4). Absorption spectra
<strong>Spectrophotometric</strong> <strong>determination</strong> <strong>of</strong> <strong>carbamazepine</strong> and mosapride<br />
citrate in pure and in pharmaceutical preparations.<br />
Eman Y.Z. Frag, M.A. <strong>Zayed</strong>*, M.M. Omar, Sally E.A. Elashery, Gehad<br />
G. Mohamed<br />
Chemistry Department, Faculty <strong>of</strong> Science, Cairo University, Gamaa Str.,<br />
12613, Giza, Egypt<br />
* Author for whom all correspondence should be addressed. (e-mail:<br />
mazayed429@yahoo.com)<br />
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