Vol. 51â1997 - NorthEastern Weed Science Society
Vol. 51â1997 - NorthEastern Weed Science Society
Vol. 51â1997 - NorthEastern Weed Science Society
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85<br />
CORRELA ION OF LABORATORY,GREENHOUSE,ANDFIELD RESPONSEOF<br />
GLVPHOSATERESISTANTTRANSGENICLETTUCE<br />
1.A. Dusky, R. T. Nagata, T. A. Bewick,R. J. Ferl, D. 1.Cantliffe'<br />
ABSTRACT<br />
Transg plants of the lettuce cultivar(cv.) 'South Bay' were producedusingtissue<br />
culture methods d seed of regeneratedplantswas obtained. To evaluatethe relativetolerance<br />
of transgenicmat rialsto glyphosate,three techniqueswere utilized;a leafdiscbioassay,a<br />
greenhouse scree . g method, and a fieldscreeningmethod. Leaf discswere obtainedfrom<br />
severaltransgeni linesby taking 1.25em leaf discsfrom the newestfullyexpandedleafof 6 wk<br />
old plants. Leaf iscs were placed on MS medium(supplementedwith NAA and BA) containing<br />
g1yphosateat v . g rates and allowedto form callusand shoots for 4 wk. The explantswere<br />
then harvested, eighed,dried and reweighed. Studieswere repeatedat least two times.<br />
Analysisofvari ce (ANOVA)was conductedto determineinteractionsand treatmenteffects.<br />
Isovalues were d terminedusing regressionanalysis.<br />
Greenho se evaluationwas done by plantingtransgeniclettuce seed in trays and allowing<br />
them to grow in greenhouseunder natural daylenthand photoperiod. <strong>Weed</strong> specieswere also<br />
seeded in trays. eeds utilizedwere bamyardgrass(Echinochloacrus-galli),lambsquarters<br />
(Chenopodiuma 'bum),spinyamaranth(Amaranthusspinosus),guineagrass(Panicum<br />
maximum), fallp nicum(Panicumdichotomiflorumy;and commonpurslane(Portulaca<br />
oleracea). After 3 wk growth, the plantswere sprayedwith glyphosateusinga CO 2 pressurized<br />
back-pack spray r. Rates utilizedrangedfrom 0 to 32.0 IbaiJA. Alltreatmentscontaineda<br />
surfactant (0.25° v/v). Two weeks after treatment,the plantswere harvested,weighed,dried<br />
and reweighed. tudies were repeatedthree times. Data were analyzedas described.<br />
Field stu ies were conductedby plantingtransgeniclettuce seed on raisedbeds. <strong>Weed</strong>s<br />
also were plant and includedspinyamaranth,fallpanicum,barnyardgrass,and yellownutsedge<br />
(Cyperus escule s). After 3 wk growth, the plantswere sprayedwith glyphosateusinga backpack<br />
sprayer. R tes ranged from 0 to 32.0 Ibai/Aand all spraymixturescontainedsurfactant.<br />
Two weeks after treatment, the plantswere harvested,dried and weighed. The experimentwas<br />
repeated twice. ata were analyzedas describedpreviously.<br />
Alltrans enic lineswere tolerant to glyphosate,althoughthere were relativedifferences<br />
between linesan the method used to evaluatetolerance. AlthoughIsovaluesfor the transgenic<br />
lettuces lineswe e differentfor each screeningtechnique,the relativetoleranceof each linewas<br />
similar. The Iso a1uesfor the weed speciesrangedfrom 0.08 to 1.741baiJAand 2.4 to greater<br />
than 8.0 lb aiJA r transgeniclettuce lines. A selectivityindexwas calculatedto determinethe<br />
relative selectivi for various transgeniclettuce linesand weed complexes.<br />
Iprof. an Assoc. Prof., EvergladesRes. & Educ. Ctr., BelleGlade,FL 33430, and Assoc.<br />
Prof', Prof', and rof., Univ. of Florida,Horticultural<strong>Science</strong>sDept., Gainesville,FL 32611.