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Vol. 51—1997 - NorthEastern Weed Science Society

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85<br />

CORRELA ION OF LABORATORY,GREENHOUSE,ANDFIELD RESPONSEOF<br />

GLVPHOSATERESISTANTTRANSGENICLETTUCE<br />

1.A. Dusky, R. T. Nagata, T. A. Bewick,R. J. Ferl, D. 1.Cantliffe'<br />

ABSTRACT<br />

Transg plants of the lettuce cultivar(cv.) 'South Bay' were producedusingtissue<br />

culture methods d seed of regeneratedplantswas obtained. To evaluatethe relativetolerance<br />

of transgenicmat rialsto glyphosate,three techniqueswere utilized;a leafdiscbioassay,a<br />

greenhouse scree . g method, and a fieldscreeningmethod. Leaf discswere obtainedfrom<br />

severaltransgeni linesby taking 1.25em leaf discsfrom the newestfullyexpandedleafof 6 wk<br />

old plants. Leaf iscs were placed on MS medium(supplementedwith NAA and BA) containing<br />

g1yphosateat v . g rates and allowedto form callusand shoots for 4 wk. The explantswere<br />

then harvested, eighed,dried and reweighed. Studieswere repeatedat least two times.<br />

Analysisofvari ce (ANOVA)was conductedto determineinteractionsand treatmenteffects.<br />

Isovalues were d terminedusing regressionanalysis.<br />

Greenho se evaluationwas done by plantingtransgeniclettuce seed in trays and allowing<br />

them to grow in greenhouseunder natural daylenthand photoperiod. <strong>Weed</strong> specieswere also<br />

seeded in trays. eeds utilizedwere bamyardgrass(Echinochloacrus-galli),lambsquarters<br />

(Chenopodiuma 'bum),spinyamaranth(Amaranthusspinosus),guineagrass(Panicum<br />

maximum), fallp nicum(Panicumdichotomiflorumy;and commonpurslane(Portulaca<br />

oleracea). After 3 wk growth, the plantswere sprayedwith glyphosateusinga CO 2 pressurized<br />

back-pack spray r. Rates utilizedrangedfrom 0 to 32.0 IbaiJA. Alltreatmentscontaineda<br />

surfactant (0.25° v/v). Two weeks after treatment,the plantswere harvested,weighed,dried<br />

and reweighed. tudies were repeatedthree times. Data were analyzedas described.<br />

Field stu ies were conductedby plantingtransgeniclettuce seed on raisedbeds. <strong>Weed</strong>s<br />

also were plant and includedspinyamaranth,fallpanicum,barnyardgrass,and yellownutsedge<br />

(Cyperus escule s). After 3 wk growth, the plantswere sprayedwith glyphosateusinga backpack<br />

sprayer. R tes ranged from 0 to 32.0 Ibai/Aand all spraymixturescontainedsurfactant.<br />

Two weeks after treatment, the plantswere harvested,dried and weighed. The experimentwas<br />

repeated twice. ata were analyzedas describedpreviously.<br />

Alltrans enic lineswere tolerant to glyphosate,althoughthere were relativedifferences<br />

between linesan the method used to evaluatetolerance. AlthoughIsovaluesfor the transgenic<br />

lettuces lineswe e differentfor each screeningtechnique,the relativetoleranceof each linewas<br />

similar. The Iso a1uesfor the weed speciesrangedfrom 0.08 to 1.741baiJAand 2.4 to greater<br />

than 8.0 lb aiJA r transgeniclettuce lines. A selectivityindexwas calculatedto determinethe<br />

relative selectivi for various transgeniclettuce linesand weed complexes.<br />

Iprof. an Assoc. Prof., EvergladesRes. & Educ. Ctr., BelleGlade,FL 33430, and Assoc.<br />

Prof', Prof', and rof., Univ. of Florida,Horticultural<strong>Science</strong>sDept., Gainesville,FL 32611.

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