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Vol. 54—2000 - NorthEastern Weed Science Society

Vol. 54—2000 - NorthEastern Weed Science Society

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POLLEN TRANSPORT FROM GENETICALLY MODIFIED CORN<br />

J.M. Jemison, Jr.' andM. Vayda'<br />

ABSTRACT<br />

Concern over the transport of genetically engineered (GE) com pollen in the U.S. is<br />

growing. Organicproducersparticularlyorganicdairyandsweet com growersfearthe<br />

loss of theirorganiccertificationif GEproteinsarefoundin theircom. Althoughcom<br />

pollen grainsare among the largest and heaviest of windpollinated plants, transport is<br />

possible. Criticsof GEfrequentlyreferto the"genecloud"of mutantcom pollen<br />

travellingdistancesof manymiles to crosspollinateandcontaminatenon-GEcom. In<br />

the sununer of 1999, we planted four research plots to com between May 15 and May 17.<br />

An 83-day Roundup-Ready (RR) com (DeKaib 335 RR) hybrid was usedfor two of these<br />

studies, andone of these studies wasvandalized in August after the com had shed pollen.<br />

These studieswere locatedsidebyside. Inthe othertwo studies,(a phosphorusfertility<br />

trial and another weed control study), we planted another 83-day hybrid, Agway 144.<br />

Becauseof the close plantingdatesandthesamematurities,thisprovidedus withthe<br />

opportunity to evaluate the potential cross pollination of GE com pollen with standard<br />

com silage hybrids.<br />

The two studies with RR com located side by side were approximately 37,190 ft' in total<br />

area and were located between the two other com trials. The phosphorus study was<br />

approximately 12,000 ft' andwas located 175 feet east of the RR study. The prevailing<br />

winds come from the southwest, andthis study should represent a worst-casescenario for<br />

cross pollination. The other weedcontrol study was located 1150 feet southwest of the<br />

RR study. The size of that plot was 11,520 ft'. This represents the lowest chance of<br />

gettingcrosspollination. To evaluatecross-pollinationandthe effect of position in the<br />

field, we divided each study into threeareas: closest to the field, a middle seetion anda<br />

seetion farthest away from the GE pollen source. Four replicate samples were collected<br />

from each study. We collected com from the RR study as well.<br />

layoutis presentedbelow.<br />

A figure showing the<br />

Com was harvested from each studyon September 17 to provide seed to germinate in the<br />

greenhouse. We collected 100 ears from each location within each study area. Com ears<br />

were dried in a greenhouse until dry enough to germinate. Seedswere sown in 4.5 ft'<br />

greenhouse flats with porting soil at half inch spacing or approximately 170 seedslflat.<br />

We are currently at this stage in theresearch. Plant emergence will be recorded for each<br />

flat. At the 2 leaf stage, plants will besprayed by hand with a 0.5% solution of Roundup<br />

Ultra. Plants that survive will be scored, andthe processwill be repeated as many times<br />

as possible to increasethe samplesize as highas possible.<br />

At this time, we have no results to present. Results will be available at the meeting.<br />

1AssociateExtensionProfessorand2ProfessorofBiochemistry- Universityof Maine<br />

8

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