Vol. 54—2000 - NorthEastern Weed Science Society

Vol. 54—2000 - NorthEastern Weed Science Society Vol. 54—2000 - NorthEastern Weed Science Society

08.06.2015 Views

ASEEDGERMrnNATION PROTOCOL FORMILE-A-MINUTE (POLYGONUMPERFOUAIUML.) 1 Wu, x: W. L. Bruckart ', R. A. Creager', and M. R. Gale 2 ABSTRACT Mile-a-minuteseedswerecollectednearBoaz,WestVirginia,in October1998.Seeds were air-driedat roomtemperaturefor 18days.Theywerethenplacedin airtightcontainersandstoredat threetemperatures:roomtemperature, 4-SoCin a refrigerator,or at -2 to _3°ein a freezer.Afterfive mooths of storage (March 1999) samples of one-hundred mature, intact seeda from each storage temperaturewere removedmonthlyandplantedin a 17x17x6em flatofBacto ProfessionalPlanting Mix (65.75% horticultura1 sphagnum peat, 34.25% perlite, pH 5.5-6.5). The planting mix was then moistenedandunsealedflatswereplacedin a refrigeratorat 4-SoCto stratifytheseeds.When seedlings emerged in any flat, all threeflats in that sample period were removed from the refrigeratorandplacedatroomtemperature.Theseflatswereobservedfortwo weeksat room temperatureandgenninationrateswererecorded.Eachflatthenwasreturnedto therefrigeratorfor additional stratification. In a separate study, one hundred seeda were planted and stratified immediately after the 18-day air-dry period. Seedling emergence wasnot observed in the refrigerator, but good emergence (86%, Figure I) resulted after moving to room temperature in March 1999. Seedsstoredatroomtemperaturebeforestratificationhadgerminationratesfrom34%to 86%, and length of stratification varied from 4 to 19 weeks. Germination rate decreased from March to September, as did the period for stratification (Figure I). Seeda stored under refrigeration had germination rates of 10'10(flat # 2-2) after 13 weeks (in June, 1999) of stratification, and 4% after 16 weeks (in July, 1999) of stratification, respectively, which were the only two months seedlings appeared. Seeda stored in the freezer had germination rates of I% after 13 weeks (in June, 1999) of stratification and 16% after 16 weeks (in July, 1999) of stratification, respectively, also the only two months seedlings appeared. New seedlings appeared in flat #2-2 in October 1999 after an additional 28 weeks ofstratitication.It'sgerminationrateincreasedby 60010to total70%overall. Germinationof Mile-a-minute seedsstoredatroomtemperaturebeforestratificationwas fasterandmorereliablethanforseedsstoredat coolertemperaturesaccordingto thisprotocol.Milea-minuteseedsmaygerminatein thefallundercontrolledcondition,basedon experiencewith flat #2-2. Plansareto continuethisstudywithseedscollectedin 1998andto initiatea new test with seeda harvested in 1999. I TheUSDA ForestService.ForestHealthTechnologyEnterpriseTeam.Morgantown,WV 26505. fundsthisresearch. 2 ResearchScientistandAssociateProfessor,Schoolof ForestryandWoodProducts,MichiganTech.Univ. Houghton, MI 49931. Thecorrespondingauthoris currentlylocatedatUSDA ForestService,ForestHeahhTechnologyEnterprise Team.Morgantown,WV 26505 3 ResearchPlantPathologistandPlantPathologistrespectively.USDA~ARS. ForeignDisease-WeedScienceResearch Unit,FortDetrick.,MD 21702 64

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