Vol. 15—1961 - NorthEastern Weed Science Society

Vol. 15—1961 - NorthEastern Weed Science Society Vol. 15—1961 - NorthEastern Weed Science Society

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560. THE ABSORPTIONANDMETABOLISMOF RADIOENDOTHAL BY FISH ANDAQUATICPLANTS1,2 V.H. Freed 3 and 1110 Gauditz 4 ABSTRACT* Endothal (disodium 3,6-endoxohexahydrophthalate)is a highly active contact weed killer that has found extensive use for pre­ emergence weed control in sugar beets, table beets, and spinach. More recently this chemical has been shown to be effective for the control of many aquatic weeds. One of the attractive features of endothal in aquatic weed control is .the relatively high tolerance that many species of fish have toward this chemical. This makes it possible to use this chemical for the control of aquatic weeds without serious effect on the fish population. The use of endothal in aquatic weed control poses the problem as to whether or not the fish would accumulate this chemical. It would be possible for the fish to ingest the chemical directly from the water or if aquatic plants accumulated the chemical, fish may become exposed to the endothal through feeding on the foliage of the aquatic plant. The purpose of the study reported here. was to . determine whether or not endothal was ingested by the fish from either source and if the endothal were taken up would it pose a problem. Previous work has shown that endothal is extremely .unstable under biological conditions So that it was felt unlikely that there would be any serious residue in the fish. A study of the uptake and distribution of C 1 4 from radioendothal in fish and plants exposed to this chemical was undertaken. It was demonstrated that the radioactivity found in goldfish exposed to different concentrations of chemical followed a similar pattern. This pattern revealed extensive breakdown of the endothal molecule and incorporation of its radioactivity into' a number offractions in both fish and plants. The behavior of these different fractions indicates that they are normal constituents of the organism such as carbohydrates, organic acids, amino acids, and proteins, as well as fats and oils. With the analytical technique used, it was demonstrated that the fraction of radioactivity which contains endothal would be the Dowex 1 eluate from the methanol extract. Even at 12 ppm this fraction contained only 0.07 ppm. At the normal range of concentration which the chemical is used, this level is only 0.002 for goldfish. Further $tudy of the elution pattern of this fraction of radioactivity indicates that it is not as endothal. Thus, it may be concluded that due to the extensive breakdown of this chemical and incorporation of radioactivity into normal conatitutents of the or-gant.sm, , .,as represented by the different fractions, that it is doubtful that any endothal remains. 1/* Complete paper to be contributed to WEEDS 2/Supported in part by a grant frem Pennsalt Chemicals Corporation, Tacoma, Washington 3/Professor, Agricultural Chemistry Department, Oregon State Col Le go 4/Present address, Hazelton Laboratory, Palo Alto, California

561. Potential Uses of Calcium Clanamid in the Control of Aquatic Vegetation J. Curtis Simes, Regional Fishery Manager Pennsylvania FiSh Commission The manuel removal of aquatic weeds from the trout rearing ponds and raceways at the Pennsylvania Fish Oommissions' Benner Spring Fish Research Station in Centre County had, prior to the 1960 season, been an operation of considerable magnitude. The dominant aquatic weeds found in these limestone waters include curly-leaved pondweed, Potamogeton crispus; commonwaterweed, Anacharis canadensis; horned pondweed, Zannichellia ~alustris; duckweed, Lemnaminor; water cress, Nasturtium officinale; and several unidentified forms of filamentous algae. At intervals averaging about once every six "Weeksduring the main growing sea son it had been necessary to cut and remove this growth from all earth-bottomed ponds and r-acevaya, Scythes and pitchforks were the usual implements employed. Substantial losses of fingerling trout frequently accompanied these manual operations. In mid-June, 1960, a number of ponds and raceways at this station were drained, scraped to remove accumulated muck and food wastes, and treated chemically with the primary objective of controlling certain fish-disease organisms. Ivlr. Gordon Trembley, Ohief Aquatic Biologist and Mr. Arthur Bradford, Fish Pathologist jointly prescribed and directed the sterilization procedures. Calcium cyanamid, a readily available and commonly used nitrogen fertilizer, was the sterilant used in roughly seventy-five percent of the treated ponds and raceways. This gray, finely-granular material was broadcast uniformly to the exposed bottoms at a rate approximating 100 pounds per 1000 square feet. Schaeperclaus (1933), the German fishery scientist, has written regarding the use of this product for fertilization and disease control in the European pond industry. Calcium hypochlorite and chlorine were the sterilsnts used in the remaining rearing facilities involved. By July 1, most ponds had been flushed and refilled with water preliminary to restocking with fish. The elapsed time between application of the sterilants and resumption of normal fish rearing operations in the various facilities ranged from a minimumof two days to a maximumof two weeks. In no case did the introduced trout appear to be adversely affected by any residual activity of the sterilants used. By late August it became apparent to some of the hatchery personnel that the cyanamid treated facilities had remained amazingly free of weed growth as opposed to those treated with the other chemical products. I~nual removal of weed growth had to be resumed in the raceways treated with calcium hypochlorite and chlorine by early September. ObserVations up to the end of October revealed that the calcium cyanamid treated areas were, with only one minor exception completely weed free. This unanticipated success in controlling or retarding aquatic weeds through the application of cyanamid to exposed bottoms prompted the initiation of several supporting experiments. To explore the potential of t.ht~ mAt,nrlAl

561.<br />

Potential<br />

Uses of Calcium Clanamid in the<br />

Control of Aquatic Vegetation<br />

J. Curtis Simes, Regional Fishery Manager<br />

Pennsylvania FiSh Commission<br />

The manuel removal of aquatic weeds from the trout rearing ponds and raceways<br />

at the Pennsylvania Fish Oommissions' Benner Spring Fish Research Station<br />

in Centre County had, prior to the 1960 season, been an operation of considerable<br />

magnitude. The dominant aquatic weeds found in these limestone waters<br />

include curly-leaved pondweed, Potamogeton crispus; commonwaterweed, Anacharis<br />

canadensis; horned pondweed, Zannichellia ~alustris; duckweed, Lemnaminor;<br />

water cress, Nasturtium officinale; and several unidentified forms of filamentous<br />

algae. At intervals averaging about once every six "Weeksduring the main<br />

growing sea son it had been necessary to cut and remove this growth from all<br />

earth-bottomed ponds and r-acevaya, Scythes and pitchforks were the usual implements<br />

employed. Substantial losses of fingerling trout frequently accompanied<br />

these manual operations.<br />

In mid-June, 1960, a number of ponds and raceways at this station were<br />

drained, scraped to remove accumulated muck and food wastes, and treated chemically<br />

with the primary objective of controlling certain fish-disease organisms.<br />

Ivlr. Gordon Trembley, Ohief Aquatic Biologist and Mr. Arthur Bradford, Fish<br />

Pathologist jointly prescribed and directed the sterilization procedures.<br />

Calcium cyanamid, a readily available and commonly used nitrogen fertilizer,<br />

was the sterilant used in roughly seventy-five percent of the treated ponds<br />

and raceways. This gray, finely-granular material was broadcast uniformly to<br />

the exposed bottoms at a rate approximating 100 pounds per 1000 square feet.<br />

Schaeperclaus (1933), the German fishery scientist, has written regarding the<br />

use of this product for fertilization and disease control in the European pond<br />

industry. Calcium hypochlorite and chlorine were the sterilsnts used in the<br />

remaining rearing facilities involved. By July 1, most ponds had been flushed<br />

and refilled with water preliminary to restocking with fish. The elapsed time<br />

between application of the sterilants and resumption of normal fish rearing<br />

operations in the various facilities ranged from a minimumof two days to a<br />

maximumof two weeks. In no case did the introduced trout appear to be adversely<br />

affected by any residual activity of the sterilants used.<br />

By late August it became apparent to some of the hatchery personnel that<br />

the cyanamid treated facilities had remained amazingly free of weed growth as<br />

opposed to those treated with the other chemical products. I~nual removal of<br />

weed growth had to be resumed in the raceways treated with calcium hypochlorite<br />

and chlorine by early September. ObserVations up to the end of October revealed<br />

that the calcium cyanamid treated areas were, with only one minor exception<br />

completely weed free.<br />

This unanticipated success in controlling or retarding aquatic weeds<br />

through the application of cyanamid to exposed bottoms prompted the initiation<br />

of several supporting experiments. To explore the potential of t.ht~ mAt,nrlAl

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