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Vol. 15—1961 - NorthEastern Weed Science Society

Vol. 15—1961 - NorthEastern Weed Science Society

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Modified<br />

Durham Sample:­<br />

An alternate meth~d ~f overcoming the directional bias<br />

is the use of a square slide in a streamlined circular holder.<br />

One of our designs allows the air to flow smo~thly over the<br />

surface, even at high speeds, as evidenced by wind tunnel<br />

tesYs. This sampler has two improvements over the standard<br />

rurham sampler: equal orientation with any air flow and<br />

:ess modification of approaching air currents. It retains<br />

~he other advantages and disadvantages.<br />

Cylinder<br />

Samplers<br />

A cylindrical surface of suitable size is a reasonably<br />

efficient collector of particles in the pollen size range at<br />

any but low wind speeds. As an added advantage, an equation<br />

has been developed for describing its efficiency mathematically.<br />

The collecting efficiency varies directly with wind speed,<br />

particle size and particle density and inversely with cylinder<br />

diameter. In sampling ragweed pollen on a cylinder of fixed<br />

size, wind speed is the only variable and a curve of collection<br />

efficiency vs. speed may be constructed. Although small<br />

cylinders are more efficient, they tend to overload too<br />

quickly for 24-hour samples, especially in dusty air, and some<br />

~ompromise between efficiency and length of the sampling period<br />

is necessary. Cylinder samplers are mounted in a vertical<br />

p~sition on vanes to keep the sampling surface oriented into<br />

the wind.<br />

A cylinder large enough to ac~ept a standard glass<br />

microscope slide in a vertical position on the leading face,<br />

without too much alteration of the cylindrical shape, should<br />

be 4 inches in diameter. This is too large for proper<br />

sampling at the usual wind speeds. To retain both the standard<br />

size slide and the smaller cylindrical shape, I-x 3-inch<br />

slides may be made of thin (~.4 rr@.) transparent plastic<br />

and attached t~ the side of a smaller cylinder (1 7lB-inch<br />

diameter) in a curved position. After a 24-hour exposure,<br />

such olastic slides will return to their original flat shape.<br />

3torage and pollen counting are essentially the same as with<br />

glass slides.<br />

A cylinder sampler with 1/4- or 3lB-inch diameter has<br />

improved efficiency at low wind speeds. This may be made ~f<br />

transparent plastic rod (luci te or pleziglass) 'Hi th a 1/16-inch<br />

wide flat portion f'or-med on the side facing t.h e wlnd. The<br />

same sticky medium (we prefer silicone gre~se) as used on<br />

the micr-os cope slides is apnLt.ed to the forv18rd face of the<br />

cylindrical rod. The pollen remains on the r~d "ltd le b~iIjg

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