Vol. 15—1961 - NorthEastern Weed Science Society

extraction was applied along the pencil line "lith a pipette drawn to a fine
point. Five applications were made tvith a period of drying between each. The
end of the paper nearest the pencil line was then inserted in a trough of the
solvent. The i'llhole was then suspended in a glass chamber. A petri dish of the
solvent was placed in the bottom of the chamber to maintain a saturated
atmosphere.' When the solvent front had advanced a suitable distance along the
paper strip, the paper was removed and, dried in a hood.
The chromatograms .were cut in'1.5 inch strips at right angles to the
movement of the solvents. For assay, sections from each strip along with 10
alfalfa seeds and 1 ml., of "rater were placed in crucible lids and covered with
watch glasses. The diameter of the crucible lid was 1.25 inches. Twocontrols
were run "lith each assay, a Water control and one from the chromatogram that
only the solvent had covered. 'Table l3'indicates the data from the assays.
The solvent'front had moved 15.5 inches. Tne numbers each represent consecutive
strips of 1. 5 inches from the starting spot. ..
347.
Table 13. Bioassay of Chromatogramed Extract
Chromatogram Section %Germina:tion Radicle lengjih from)
Check - water 90 17
Check - solvent 95 10
1 90 7
2 0 0
3' 90 6
4 100 6
5 70 11
6 100 10
7 60 3
B 90 9
9 100 14
10 60 s
Areas'2 and 7 reryresent regions of strong inhibition tvith Rf values
of 0;13 and 0;75. Areas 5 and 10 exhibited slight inhibition ,'lith Rf' values
of 0.40 and 0.90. A section of the chromatogram was develoned for a general
sugar test. The test consisted of:
a. Silver nitrate saturated water 0.1 vol.,
. Acetone . 20 vol.
b. Sodium hyrlroxide, 0.5% in ethanol