Vol. 15—1961 - NorthEastern Weed Science Society

Vol. 15—1961 - NorthEastern Weed Science Society Vol. 15—1961 - NorthEastern Weed Science Society

08.06.2015 Views

320. growing season in the 1957-59 study, fall treatments were not included in the 1959~60 experiment. The experimental design of the treatments applied was a 3 x 6 splitplot factorial as fo,Hows: ~1hole plots---Cultural treatments A - Plow B - Fallow ' C- .~ cultivation r.' Split plots---bhemical treatments' 1 - Atrazine 2 - Stmazine 3 - /.mitrol-1 4 - Penac 5 - Dalapon 6 - No herbicide All possible combinations of the treatments were replicated four times, makings total of 12 plots •. . The herbicides Were all applied on May 16, 1959 at the rate of eight Ibs. per acre in 30 gallons of water. All plots except those which were to receive no cultural treatment were plowed on June 2, 1959. The plots to be fallowed were disked or harrowed with a spring-tooth harrow frequently during the growing season to prevent growth and photosythesis. " Samplesofquackgraas rhizomes were taken periodically for laboratory analysis according to the folloWing schedule: June IS, 1959 Auaust 5, 1959 ·September 10, 1959 November 10,1959 Hay 10, 1960 A steel cylinder having an area of one square foot was used to obtain the samples •. An area in each plot which had not been previously sampled W4S selected at random and all weeds and topgrowth of quackgrass was removed. The cylinder having sides constructed of 3/8 inch thick steel and 12 inches high, with a one inch steel plate top, was placed over this area and pounded into the soil to a depth sufficient to obtain all rhizomes. After all soil and foreign material were removed, the clean rhizomes from each plo'twer,e . placed in a paper bag and dried in a forced"'alr oven at 70· C. After drying. the rhizomes were weighed, ground and stored in airtight bottles until analysis' was to be made. They were then redried, mixed thoroughly and analyzed for carbohydrate content. The procedure used in the quantitative determinations of carbohydrate was the same as preViously reported for the 1957·59 experiment ( 1 ). which was bas&d!on a colored compound formed by the interaction of~f!ucto~e and resorcinol.

On the basis of the rhizomes obtained from one square foot of soil per plot on each sampling date, the yields of rhizomes and fructose per unit area, as well as the concentration of total fructose, were determined. This paper deals only with the main effects of and interaction between chemical and cultural treatments on the fructose content of the quackgrass rhizomes. RESULTSANDDISCUSSION The main effects of both cultural and chemical treatment's, as well as their interaction, resulted in significant differences in the rhizome reserves at the one per cent level throughout the experiment. Main effects of cultural treatments: The main effects of the cultural treatments on the carbohydrate content of quackgrass rhizomes are given in Table I. Both spring ploWing and fallow resulted in rapid decreases of about 50 per cent in fructose content. The single spring ploWing treatment continued to hold the rhizomes near this low level of carbohydrates until late autumn, when sufficient regrowth had taken place to again accumulate photosynthate in the underground organs. It still showed a 15 per cent reduction over the uncultivated plots, but this difference was no longer significant. The data from the final sampling the following spring showed the fructose content from the plowed rhizomea to be almost equal to the uncultivated rhizomes. On the other hand, the continuous fallow treatment continued to deplete the rhizomes of their stored food, resulting in depletions of about 70 per cent toward fall of 1959 and 74 per cent by May 10, 1960. The data on fructose content over all sampling dates show that the fluctuations in the per cent fructose of normal untreated rhizomes are small, with the reserves remaining at a high level from early' summer to late autumn. Winter respiration and early spring regrowth, however, caused a decrease in the fructose content of about 50 per cent as seen from the data of May 10, 1960. Main effects of chemical treatments: The main effects of the herbicides on the carbohydrate content of quackgrass rhizomes are given in Table II. Atrazine was most striking in its effect on the underground reserves, not only based on its rate and extent of reduction but also on its permanency. It resulted in a very sharp and early decrease of 71 per cent in the fructose content at the first sampling date. Its effect continued to increase slowly until the carbohydrates had been depleted by 83 per cent by late autumn and -..' 90 per cent by the following spring. The effect of Simazine on the fructose content was somewhat slower, resulting in a decrease of 54 per cent on June IS, but was almost equal to 321.

On the basis of the rhizomes obtained from one square foot of soil per<br />

plot on each sampling date, the yields of rhizomes and fructose per unit<br />

area, as well as the concentration of total fructose, were determined. This<br />

paper deals only with the main effects of and interaction between chemical<br />

and cultural treatments on the fructose content of the quackgrass rhizomes.<br />

RESULTSANDDISCUSSION<br />

The main effects of both cultural and chemical treatment's, as well as<br />

their interaction, resulted in significant differences in the rhizome reserves<br />

at the one per cent level throughout the experiment.<br />

Main effects of cultural treatments:<br />

The main effects of the cultural treatments on the carbohydrate content<br />

of quackgrass rhizomes are given in Table I.<br />

Both spring ploWing and fallow resulted in rapid decreases of about<br />

50 per cent in fructose content. The single spring ploWing treatment continued<br />

to hold the rhizomes near this low level of carbohydrates until late<br />

autumn, when sufficient regrowth had taken place to again accumulate photosynthate<br />

in the underground organs. It still showed a 15 per cent reduction<br />

over the uncultivated plots, but this difference was no longer significant.<br />

The data from the final sampling the following spring showed the fructose<br />

content from the plowed rhizomea to be almost equal to the uncultivated<br />

rhizomes.<br />

On the other hand, the continuous fallow treatment continued to deplete<br />

the rhizomes of their stored food, resulting in depletions of about 70 per<br />

cent toward fall of 1959 and 74 per cent by May 10, 1960.<br />

The data on fructose content over all sampling dates show that the<br />

fluctuations in the per cent fructose of normal untreated rhizomes are small,<br />

with the reserves remaining at a high level from early' summer to late autumn.<br />

Winter respiration and early spring regrowth, however, caused a decrease in<br />

the fructose content of about 50 per cent as seen from the data of May 10,<br />

1960.<br />

Main effects of chemical treatments:<br />

The main effects of the herbicides on the carbohydrate content of quackgrass<br />

rhizomes are given in Table II.<br />

Atrazine was most striking in its effect on the underground reserves,<br />

not only based on its rate and extent of reduction but also on its permanency.<br />

It resulted in a very sharp and early decrease of 71 per cent in the fructose<br />

content at the first sampling date. Its effect continued to increase slowly<br />

until the carbohydrates had been depleted by 83 per cent by late autumn and<br />

-..' 90 per cent by the following spring.<br />

The effect of Simazine on the fructose content was somewhat slower,<br />

resulting in a decrease of 54 per cent on June IS, but was almost equal to<br />

321.

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