Vol. 16—1962 - NorthEastern Weed Science Society

237
Figure I Bioassay of cbrOlll4tographed extflact
,
~ick
------_.
__.__._.. _._._._--_._.-_.
0
Extract Streak 0
-I
I
--Methyl
Red streak
~=i
0
0
0
Methyl Red 0
0
0
0
Yellow 0 I
0
1
0
Front 0
0 ,I
0
I
i
Rf
Control
o
9
17
24
31
37
43
48
57
64
71
77
84
92
98
Percent
Inhibition
o
16
-I
-4
54
-9
16
14
-21
100
60
80
77
22
-27
-31
11
Summaryand Conclusions
Agueous extracts of 5/16 g. Artemesia vulgaris when diluted to 100 mI.
inhibited alfalfa seedling growth over 50%. The inhibitor was found in green
and in dry plant material and could be extracted from either leaves, roots, stems,
or flowers. It was stable to autoclaving at 20 p.s.i. for 20 minutes, but was
destroyed completely when ashed. It was dialyzable and nonvolatile. It was
soluable in polar and semipolar solvents and partly soluable in some nonpolar
solvents. Inhibition e:~tracted with nonpolar solvents could be removed by
shaking with water in a separatory funnel. The substance was not absorbed to
either strong cation or anion exchange resins. Paper chromotographs run in
isopropanol and water (90 - 10 /) in an IIIIIIIIOniatmosphere showed the inhibitor
or inhibitors to have an Rf value between 56 and 76.
Literature
cited
Bonner, J. 1950. The role of toxic substances in the interaction of higher
plants. Bot. Rev. 16:51-65.
Borner, H. 1960. Liberation of organic substances from higher plants and their
role in the soil sickness problem. Bot. Rev. 26: 393-424.