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Vol. 16—1962 - NorthEastern Weed Science Society

Vol. 16—1962 - NorthEastern Weed Science Society

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234<br />

we:re used .1n tbe .:r8ll!&1nde:rof tq::@tW\y.<br />

Effect<br />

of "beat<br />

'J<br />

,';,- " .<br />

When a coiuam packed witb'~:'drYed meal wasa~to~lav.d b.fore ext:rac~icm.<br />

tbere was no loss of inhibition (Table 3).<br />

I&:able.3. Effecit,o~ aut!clcv:1nl on inhlb1t~ of<br />

a1£alf,a seed1iaa ..gJ:01l1th.<br />

.......<br />

------_._<br />

.;.;.-.._._.:.<br />

---.. _~,~.'.._-_. ---...-..._-..,..'.'-.~..;.-.._..... _..-..•;.....<br />

• '1_<br />

Percent Inhibition<br />

Concentration * 5g ./100 ml. 2% g ./100 "g./~OO 518 g./1oo .. 5/].6/100<br />

..----_ --•.•..••... _-.._-- - - -- ---_..-.-_ ..----~.<br />

Autoc1aved<br />

100<br />

87<br />

89 71<br />

Non-Autoc1aved<br />

88<br />

90<br />

85 71 56<br />

<strong>Vol</strong>atile<br />

-17 .<br />

-_..--_.-_.-_.•-.••••.••••---jllt ••••--.•••--_..•••_-~.--••••••.•.•_-_ .<br />

* See footnote Table 1<br />

This showed that the<br />

'.<br />

inhibitor is<br />

.,-,<br />

.8t.le at 1200 C _2~ peifor 20 minutes.<br />

When'the first 100 iDl. passecn:brough anautoc"!.ved column ltwas .vaporated<br />

and the wa,te:r,cODClencedunde:r, _ ....oe pack. The ... ay showed alfalfa seedling<br />

growth slishtly. peatier tban ~t, pf the controls. It is evident from this<br />

e:cper:lment that the active principle 1.s non·vol.tile.· ..<br />

Soxhlet<br />

extraction<br />

AcOiDparison of extractionefflCiency of severa"!' solvents was made ualng<br />

the' Soalet ext:ractor. Art_s1., vuJ,8ari! llleal wu.ti:aClted 48 hours.<br />

.-.._.....-.-_.-<br />

Table 4 Soxhlet Extr.~JI;~D<br />

.....__._...-•.._..._.~~..~_....<br />

_..-....._..<br />

Percent<br />

Percent<br />

. . gerad:ution . inhibtttea '. .<br />

._-_.---._-_..------_._--...__...._-_._-_._..-~~..~_..-.... '<br />

Control<br />

Water<br />

Methanol<br />

Acetone<br />

Eenzetle<br />

Xylene<br />

Pet Ether<br />

Chlorofo:rm<br />

95<br />

00<br />

15<br />

4'5"<br />

85<br />

95<br />

9S<br />

50<br />

'00<br />

100 u<br />

79<br />

8!l<br />

68'<br />

13'<br />

16<br />

71<br />

The solvents were evaporated and the :residu.s shaken with water with a wrist<br />

action shaker for 20 minutes.<br />

The results showed, the inhib1.tton was most sollable in non·polar solvents<br />

(Table 4) • However. there were 1.Dbibttory substaucuJ extracted, by some of'the

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