Tese_Tânia Vieira.pdf - Ubi Thesis

% of viable cells
% of viable cells
% of viable cells
% of viable cells
Chapter III – Results and Discussion
between themselves and the K + . For the supernatant of the nanoparticles there was no
significant difference between themselves and the K + , demonstrating that the supernatant was
toxic for the cells.
Lower concentration
MIC
150
100
50
#
# # #
# # # *#
* * * * * * *
24 h
48 h
150
100
50
#
# #
#
#
#
*#
* * * * * * *#
24 h
48 h
0
1 2 3 4 5 6 7 K- K+
0
1 2 3 4 5 6 7 K- K+
Higher concentration
Supernatant
150
150
100
50
* # * # * # *
# *#
#
*
*
*
24 h
48 h
100
50
* #
24 h
48 h
0
1 2 3 4 5 6 7 K- K+
0
1 2 3 4 5 6 7 K- K+
Figure 15 –Cell viability measured by the MTS assay after 24 and 48 h in contact with chitosan/dextran
nanoparticles (1); chitosan/dextran nanoparticles with AgNPs (NaBH 4 ) (2); chitosan/dextran nanoparticles
with AgNPs (C 6 H 8 O 6 ) (3); chitosan/dextran nanoparticles with AgNO 3 and with NaBH 4 (4); chitosan/dextran
nanoparticles with AgNO 3 and with C 6 H 8 O 6 (5); AgNPs with NaBH 4 (6); AgNPs with C 6 H 8 O 6 (7); in lower
concentrations (A), at MIC concentrations (B) and in higher concentrations (C). The cellular activity was also
measured for supernatants of the referred nanoparticles after 24 and 48 h (D). Negative control (K - ) and
positive control (K + ) are also presented for both cases. Each result is the mean ± standard error of the mean
of at least three independent experiments. Statistical analysis was performed using one-way ANOVA with
Bonferroni t-test (* , # p