Tese_Tânia Vieira.pdf - Ubi Thesis
Tese_Tânia Vieira.pdf - Ubi Thesis
Tese_Tânia Vieira.pdf - Ubi Thesis
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
% of viable cells<br />
% of viable cells<br />
% of viable cells<br />
% of viable cells<br />
Chapter III – Results and Discussion<br />
between themselves and the K + . For the supernatant of the nanoparticles there was no<br />
significant difference between themselves and the K + , demonstrating that the supernatant was<br />
toxic for the cells.<br />
Lower concentration<br />
MIC<br />
150<br />
100<br />
50<br />
#<br />
# # #<br />
# # # *#<br />
* * * * * * *<br />
24 h<br />
48 h<br />
150<br />
100<br />
50<br />
#<br />
# #<br />
#<br />
#<br />
#<br />
*#<br />
* * * * * * *#<br />
24 h<br />
48 h<br />
0<br />
1 2 3 4 5 6 7 K- K+<br />
0<br />
1 2 3 4 5 6 7 K- K+<br />
Higher concentration<br />
Supernatant<br />
150<br />
150<br />
100<br />
50<br />
* # * # * # *<br />
# *#<br />
#<br />
*<br />
*<br />
*<br />
24 h<br />
48 h<br />
100<br />
50<br />
* #<br />
24 h<br />
48 h<br />
0<br />
1 2 3 4 5 6 7 K- K+<br />
0<br />
1 2 3 4 5 6 7 K- K+<br />
Figure 15 –Cell viability measured by the MTS assay after 24 and 48 h in contact with chitosan/dextran<br />
nanoparticles (1); chitosan/dextran nanoparticles with AgNPs (NaBH 4 ) (2); chitosan/dextran nanoparticles<br />
with AgNPs (C 6 H 8 O 6 ) (3); chitosan/dextran nanoparticles with AgNO 3 and with NaBH 4 (4); chitosan/dextran<br />
nanoparticles with AgNO 3 and with C 6 H 8 O 6 (5); AgNPs with NaBH 4 (6); AgNPs with C 6 H 8 O 6 (7); in lower<br />
concentrations (A), at MIC concentrations (B) and in higher concentrations (C). The cellular activity was also<br />
measured for supernatants of the referred nanoparticles after 24 and 48 h (D). Negative control (K - ) and<br />
positive control (K + ) are also presented for both cases. Each result is the mean ± standard error of the mean<br />
of at least three independent experiments. Statistical analysis was performed using one-way ANOVA with<br />
Bonferroni t-test (* , # p