Tese_TÃ¢nia Vieira.pdf - Ubi Thesis

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Chapter III – Results and Discussion Table 5 – Different nanoparticles and their concentrations to perform the cytotoxic assays. Low Nanoparticles Identification concentration (µg/mL) MIC (µg/mL) High concentration (µg/mL) 1 Chitosan/dextran nanoparticles 20.0000 107.1000 428.6000 2 3 4 5 Chitosan/dextran nanoparticles with AgNPs (NaBH 4) Chitosan/dextran nanopartices with AgNPs (C 6H 8O 6) Chitosan/dextran nanoparticles with AgNO 3 and NaBH 4 Chitosan/dextran nanoparticles with AgNO 3 and C 6H 8O 6 0.3000 1.5000 6.0000 0,1875 3.0000 6.0000 1,6243 11.7500 42.5000 1,6243 23.5000 42.5000 6 AgNPs (NaBH 4) 2,5000 10.0000 40.0000 7 AgNPs (C 6H 8O 6) 2,5000 10.0000 40.0000 Cell adhesion and proliferation was observed by using an inverted light microscope after 24 and 48 h (figure 13 and 14). In the negative control (K - ) cells were viable, appearing with stellate geometry and showing slender lamellar expansions that joined neighboring cells. In the positive control (K + ), dead cells were seen with their spherical characteristic form. For the cells in contact with the different concentrations of nanoparticles, no toxic effects were observed when nanoparticles in low or MIC concentrations were put in contact with cells during 24 and 48 h. This can be depicted in figure 13, where cells were similar to that of the K + . For the high concentrations of nanoparticles almost no toxic effects were obtained, except for the particles presented in figure 14 A, 4 (Chitosan/dextran with AgNO 3 and with C 6 H 8 O 6 as reducing agent) and 5 (Chitosan/dextran with AgNO 3 and with NaBH 4 as reducing agent) that presented some dead cells. In the figure 14 B are presented the results obtained for the cells in contact with the supernatants obtained from the nanoparticles washing, in which some adhered cells and some dead cells were observed, indicating some toxic effects on the cells, during the period of exposure to the supernatants. 44
Chapter III – Results and Discussion A B Figure 13 – Inverted Light Microscope Images of human osteoblast cells in contact with: chitosan/dextran nanoparticles (1); chitosan/dextran nanoparticles with AgNPs produced with NaBH 4 (2); chitosan/dextran nanoparticles with AgNPs produced with C 6 H 8 O 6 (3); chitosan/dextran nanoparticles with AgNO 3 and with NaBH 4 (4); chitosan/dextran nanoparticles with AgNO 3 and with C 6 H 8 O 6 (5); AgNPs produced with NaBH 4 (6); AgNPs produced with C 6 H 8 O 6 (7); in lower concentrations (A) and at MIC concentrations (B) after 24 and 48 h. Negative control (K -) and positive control (K + ) are also presented for both cases. (×10) 45
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UNIVERSIDADE DA BEIRA INTERIOR Ciê
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Acknowledgments First of all, I wou
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Table of Contents 1. Introduction .
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Page 16 and 17: List of Tables Chapter I - Introduc
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Page 21 and 22: Chapter I Introduction
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Page 25 and 26: Chapter I - Introduction Due to the
Page 27 and 28: Chapter I - Introduction materials
Page 29 and 30: Chapter I - Introduction Besides it
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Page 33 and 34: Chapter I - Introduction ROS produc
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Page 37 and 38: Chapter I - Introduction Furthermor
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Page 41 and 42: Chapter II - Materials and Methods
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Page 79 and 80: Bibliography Pal, S., Tak Y.K. and
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