Tese_TÃ¢nia Vieira.pdf - Ubi Thesis

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Chapter I - Introduction biomedical and pharmaceutical applications (Carmen Rodríguez-Argüelles et al. 2011). Furthermore, it also presents very important biological properties among which antimicrobial, anti-inflammatory, and antioxidant (Carmen Rodríguez-Argüelles et al. 2011). Figure 4 – Representation of the chitosan chemical structure(Kumirska et al. 2011). In this work, the chitosan aqueous solution was added to dextran sulfate polyanion aqueous solution. The formation of polycation–polyanion (polyelectrolyte) complex was mainly driven by an electrostatic mechanism where charge neutralization and possible local bridging (such as hydrogen bounding, Coulomb forces, van der Waals forces, and transfer forces) occurs (Yu et al. 2005; Meng et al. 2010). The advantages of chitosan/dextran nanoparticles are enhanced stability and increased mechanical strength compared with chitosan/tripolyphosphaste microparticles, whose lower stability and mechanical strength limit their application for drug delivery (Chen et al. 2007). It has also been reported that DNA and insulin structures are protected when dextran is used in the formulation of polyethylenimine/dextran nanoparticles (Chen et al. 2007). Dextran was also described as being capable of reducing the cationic chargerelated cytotoxicity of PEI nanoparticles in vitro (Chen et al. 2007). Therefore, it is possible that the combination of chitosan and dextran as matrix materials, in an optimal charge ratio, may act synergistically to incorporate and protect proteins and drugs, in order to reduce the toxicity of chitosan caused by its cationic charge (Kean et al. 2010). 18
Chapter I - Introduction 1.4. Objectives In the present study different nanoparticles with antibacterial properties were produced in order to be applied in several biomedical products like bone implants or skin substitutes. The specific objectives of the workplan herein presented are the following: - Development of AgNPs stabilized with chitosan/dextran; - Determination of the antibacterial activity of the produced nanoparticles; - Characterization of the different nanoparticles by different methods: Fourier Transform Infrared (FT-IR), Ultraviolet-Visible (UV-Vis), and Scanning Electron Microscopy (SEM); - Evaluation of the cytotoxic profile of the nanoparticles. 19
Page 1 and 2: UNIVERSIDADE DA BEIRA INTERIOR Ciê
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Page 5: Acknowledgments First of all, I wou
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Page 11 and 12: Table of Contents 1. Introduction .
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Page 16 and 17: List of Tables Chapter I - Introduc
Page 18 and 19: List of Acronyms Ag 0 Metallic silv
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Page 23 and 24: Chapter I - Introduction al. 2011).
Page 25 and 26: Chapter I - Introduction Due to the
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Page 33 and 34: Chapter I - Introduction ROS produc
Page 35 and 36: Chapter I - Introduction central is
Page 37: Chapter I - Introduction Furthermor
Page 41 and 42: Chapter II - Materials and Methods
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Page 71 and 72: Chapter IV - Conclusions and Future
Page 73 and 74: Bibliography 5. Bibliography Albers
Page 75 and 76: Bibliography Fernebro, J. (2011). "
Page 77 and 78: Bibliography Li, W.R., Xie X.B., Sh
Page 79 and 80: Bibliography Pal, S., Tak Y.K. and
Page 81 and 82: Bibliography Speranza, G., Gottardi

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