Tese_Tânia Vieira.pdf - Ubi Thesis
Tese_Tânia Vieira.pdf - Ubi Thesis
Tese_Tânia Vieira.pdf - Ubi Thesis
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List of Figures<br />
Chapter I – Introduction<br />
Figure 1 – Applications of silver and AgNPs in medicine. ................................................. 10<br />
Figure 2 – Different mechanisms of action of AgNPs against bacteria.................................. 13<br />
Figure 3 – Representation of the dextran chemical structure. .......................................... 17<br />
Figure 4 – Representation of the chitosan chemical structure .......................................... 18<br />
Chapter III – Results and Discussion<br />
Figure 5 – SEM images of AgNPs ............................................................................... 28<br />
Figure 6 – SEM images of chitosan/dextran nanoparticles produced with low molecular weight<br />
chitosan............................................................................................................ 29<br />
Figure 7 – SEM images of chitosan/dextran nanoparticles produced with high molecular weight<br />
chitosan............................................................................................................ 31<br />
Figure 8 – SEM images of chitosan/dextran nanoparticles with AgNPs and AgNPs produced in<br />
chitosan/dextran nanoparticles with NaBH 4 and with C 6 H 8 O 6 . .......................................... 33<br />
Figure 9 – Ultraviolet-Visible spectra of the produced AgNPs ........................................... 35<br />
Figure 10 – FT-IR spectra of chitosan, dextran, and chitosan/dextran nanoparticles ............... 37<br />
Figure 11 – FT-IR spectra of chitosan/dextran nanoparticles with AgNPs and AgNPs produced in<br />
chitosan/dextran nanoparticles with NaBH 4 and with C 6 H 8 O 6 ........................................... 38<br />
Figure 12 – Determination of MIC and MBC by the microdilution method in microplate. ........... 40<br />
Figure 13 – Inverted Light Microscope Images of human osteoblast cells in contact with<br />
nanoparticles of lower and MIC concentrations after 24 and 48 h .................................... 45<br />
Figure 14 – Inverted Light Microscope Images of human osteoblast cells in contact with<br />
nanoparticles of higher concentrations and their supernatants after 24 and 48 h .................. 46<br />
Figure 15 – Cellular activities measured by the MTS assay after 24 and 48 h in contact with<br />
nanoparticles in lower, MIC, higher concentrations and their supernantants ....................... 48<br />
xiv
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