Diagnosing Strawberry Root and Crown Diseases - Santa Barbara ...

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Soil Borne Pathogens – other methods • ELISA: Enzyme-linked Immunosorbent Assay • No need to culture and gives very rapid results • Very sensitive to low pathogen density • Doesn’t require the pathogen to be alive (+/-) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked secondary antibody is added, and binds to detecting antibody; (5) substrate is added, and is converted by enzyme to detectable form (color change)
Soil Borne Pathogens – other methods • ELISA: Enzyme-linked Immunosorbent Assay • Quick tests for some select pathogens (mostly viruses) • Can be used in the field – give rapid results but maybe only to genus (Phytophthora), sometimes to species (X. fragariae)
Page 1 and 2: Diagnosing Strawberry Root and Crow
Page 3 and 4: Soil Borne Pathogens • Microscopy
Page 5 and 6: Soil Borne Pathogens- Culturing •
Page 7: Soil Borne Pathogens- Culturing •
Page 11 and 12: Most Common Strawberry Root and Cro
Page 13 and 14: Anthracnose Crown and Root Rot •
Page 15 and 16: Cylindrocarpon Black Root Rot • C
Page 17 and 18: Fusarium oxysporum • Easy to isol
Page 19 and 20: Charcoal Rot - Macrophomina phaseol
Page 21 and 22: Phytophthora Crown and Root Rot •
Page 23 and 24: Verticillium Wilt • Verticillium
Page 25 and 26: Testing soil pre-plant for Verticil
Page 27 and 28: Strawberry Nematodes: Soil-borne en

Diagnosing Strawberry Root and Crown Diseases - Santa Barbara ...

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