Affinity Chromatography - Department of Molecular and Cellular ...

Appendix 2
Selection of purification equipment
Many affinity chromatography experiments may be carried out using the simplest methods
and equipment, for example step-gradient elution using a syringe together with prepacked
HiTrap columns. When more complex elution methods are necessary, large sample volumes
are being applied or the same column is to be used for many runs in series, it is wise to use
a dedicated system.
Standard ÄKTAdesign configurations
Explorer Purifier FPLC Prime Syringe+ Centrifugation+
Way of working 100 10 HiTrap MicroSpin
Rapid, screening (GST or
His tagged proteins) "
Simple, one step
purification " " " " "
Reproducible performance
for routine purification " " " "
Optimization of one
step purification to
increase purity " " " "
System control and data
handling for regulatory
requirements, e.g. GLP " " "
Automatic method
development and
optimization " " "
Automatic buffer
preparation " "
Automatic pH scouting " "
Automatic media or
column scouting "
Automatic multi-step
purification "
Scale up, process
development and
transfer to production "
ÄKTAprime
ÄKTAFPLC
ÄKTAexplorer
ÄKTApurifier
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