Affinity Chromatography - Department of Molecular and Cellular ...

This handbook describes the role of affinity chromatography in the purification of
biomolecules, the principle of the technique, the media available and how to select them,
application examples and detailed instructions for the most commonly performed procedures.
Practical information is given as a guide towards obtaining the best results.
The illustration on the inside cover shows the range of handbooks that have been produced
by Amersham Pharmacia Biotech to ensure that purification with any chromatographic
technique becomes a simple and efficient procedure at any scale and in any laboratory.
Symbols and abbreviations
this symbol indicates general advice which can improve procedures or provide
recommendations for action under specific situations.
this symbol denotes advice which should be regarded as mandatory and gives a warning
when special care should be taken.
this symbol highlights troubleshooting advice to help analyse and resolve difficulties that
may occur.
chemicals, buffers and equipment.
experimental protocol.
PBS phosphate buffered saline (140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 ,
1.8 mM KH 2 PO 4 , pH 7.4).
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