Egyptian Journal <strong>of</strong> Medical Microbiology, January 2008 Vol. 17, No. 1 DISCUSSION Epidemiologic studies <strong>of</strong> <strong>tuberculosis</strong> can be greatly facilitated by the application <strong>of</strong> strainspecific markers. The discoveries <strong>of</strong> transposable elements in <strong>Mycobacterium</strong> <strong>tuberculosis</strong> have been shown to be <strong>of</strong> great potential for use in strain identification. This study represents early attempts at tracking diversity particularly among drugresistant strains consistent with low-incidence countries. In this study we characterized <strong>Mycobacterium</strong> <strong>tuberculosis</strong> <strong>complex</strong> (MTC) isolates from sputum <strong>of</strong> patients infected with MTB from Egypt in order to establish a database <strong>of</strong> strain types and antimicrobial susceptibility patterns. Among the 45 MTC isolates from sputum samples obtained from Suez Canal Hospitals in Egypt, 44 were identified as MTB and one as M. bovis by PCR-RFLP analysis <strong>of</strong> oxyR. This finding suggests that M. bovis plays a minor role, compared to MTB, in the etiology <strong>of</strong> pulmonary <strong>tuberculosis</strong> in Egypt. We found 40 IS6110 RFLP patterns among the 44 MTB isolates tested. None <strong>of</strong> these patterns may be considered low-copy number (
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