Strain differentiation of Mycobacterium tuberculosis complex ...

Egyptian Journal of Medical Microbiology, January 2008 Vol. 17, No. 1
Table 2. RFLP types according to the antimicrobial susceptibility patterns of the 44 tested M. tuberculosis
isolates from sputum samples of pulmonary tuberculosis patients in Egypt
Resistance pattern Number of isolates RFLP patterns
INH 1 32
STR 4 22, 30, 5, 16
RIF + INH 3 6, 15, 38
INH + STR 3 2,5,17
INH+RIF+STR 1 3
INH+RIF+EMB 1 39
INH+RIF+ STR +EMB 6 7,9,10,20,21,40
None 25 Multiple *
Total 44
* 21 RFLP patterns.
Table 3. Spoligotypes of 44 MTB isolates from sputum of patients with pulmonary tuberculosis in Egypt.
Spoligotype
Number of
Isolates
Spoligotype a
RFLP type (s)
A b 12 777777777760771 1, 21 c , 25, 26 c , 27, 28 c ,38,39,40
B 1 777777367720771 2
C 2 777777377760771 9, 3
D 1 777777777740171 4
E 2 477677577760771 6, 19
F b 1 377777607760771 5
G 3 437777777760771 20, 7, 10
H b 4 777777607760771 8, 12, 32, 33
I b 2 703777740003171 31, 11
J b 1 776377777760771 13
K 2 777777607760751 14
L 1 037637767760771 15
M 2 770000777760771 16, 17
N 1 703777740003771 22
O 1 703777600003171 29
P 1 776367777760771 30
Q 1 777777601760771 24
R 1 777777347760471 35
S 2 777777777620771 23, 34
T 1 677777777413731 37
U 1 776377740000031 36
V 1 777737607760771 18
Total 44 40
a, 15-digital octal code (REF).
b, Types were found among Egyptian isolates isolates in previous studies.
c, each FRLP pattern contain 2 isolates.
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