WET LAB DNA Barcoding: From Samples to Sequences - Northwest ...

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WET LAB KEY • Skin (if they contain the basal layer of the epidermis, which gives rise to new skin cells; the topmost layers of skin cells or stratum corneum are dead and contain little DNA) • Fish scales If students have learned about blood cells in their classes, they should know that red blood cells do not have nuclei and therefore are not a source of DNA. However, whole blood also contains white blood cells, which are often used for DNA purification. Using Bioinformatics: Genetic Testing 368 ©Northwest Association for Biomedical Research—Updated October 2012
WET LAB KEY Lab 2: Copying the DNA Barcoding Gene Using Polymerase Chain Reaction (PCR) Teacher Answer Key On your separate sheet of paper or in your lab notebook, answer each of the following questions: 1. What did you do in this laboratory experiment and why? Copied the DNA barcoding gene, COI, from the purified DNA obtained in Laboratory Experiment 1. This PCR product will be used in subsequent experiments, including agarose gel electrophoresis and DNA sequencing. 2. What skills did you learn or practice? This question is designed to help students identify laboratory skills that they can list on a resume and/or college application. • Handling samples • Pipetting • Performing a Polymerase Chain Reaction 3. What are we testing for in the Negative Control PCR? What do you expect to happen in this PCR? Students are adding only water to their Negative Control PCR – no DNA. They are testing for DNA contamination of any of their PCR reagents, such as the primer pool and/or the nuclease-free water. They do not expect anything to happen in this PCR as there should not be any DNA present to copy. Wet Lab – DNA Barcoding: From Samples to Sequences 369 ©Northwest Association for Biomedical Research—Updated October 2012
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