Final Exam—May 4, 2005 Multiple Choic

Name_____________________________
Biotechnology, Society and the Environment (Agri 1501)
Final Exam—May 4, 2005
Multiple Choice. Choose the best answer for each of the following questions.
(2 pts./question; 48 pts. total).
1. Capable of making an entire organism
a. totipotent
b. impotent
c. pluripotent
d. potent
2. In the PCR reaction, double stranded DNA is denatured into single strands at
a. 40 0 C
b. 58 0 C
c. 72 0 C
d. 94 0 C
3. What is your diploid number of chromosomes?
a. 23
b. 46
c. 92
d. 1
4. Adenovirus vector systems do the following:
a. home to the pancreas
b. home to the lungs
c. can be used in gene therapy to treat cystic fibrosis
d. are retroviruses
e. both b and c
f. both a and c
5. DNA sequences that are expressed can be
a. junk DNA
b. STRs
c. open reading frames that encode proteins
d. repeated satellite DNA
6. Type 1 Diabetes is the result of
a. immunorejection
b. autoimmunity
c. insulin resistance
d. fasting glucose

7. A person that has an autosomal recessive disease
a. is a carrier
b. actually has the disease
c. will never get the disease
d. can only produce offspring with the disease
e. both b and d
8. Retroviral gene therapy works for brain tumors because
a. they only infect replicating tumor cells, not non-dividing brain cells
b. they infect only non-dividing brain cells
c. only retrovirally infected tumor cells will be killed by a drug specific for a
gene that the viral is carring
d. brains generally like retroviruses
e. both b and c
f. both a and c
9. Therapeutic cloning is
a. allowed in certain states if NO Federal funds are used
b. is a way to produce an individual’s own ES cells as future spare parts
c. is guaranteed to make babies
d. both b and d
e. both a and b
10. The mitotic phase of the cell cycle is also called the
a. phase of the moon
b. S phase
c. G 1 phase
d. M phase
e. G 2 phase
11 A way to contain transgenic fish
a. make all females sterile
b. use smarter worms and sharper hooks
c. use land-locked holding ponds as currently in place for catfish
d. both a and c
e. both b and c
12. A short repeated polymorphism that can identify 1 person out of 10 18 by
PCR
a. SRP
b. STR
c. EST
d. PGC

13. Finding the CFTR gene utilized the following methods
a. reverse genetics
b. linkage analysis
c. forward genetics
d. whole genome sequencing
e. both a and b
f. both b and d
14. DNA that has been immobilized on a membrane for analysis with probes
a. Western blot
b. Northern blot
c. Eastern blot
d. Southern blot
15. The rapidly expanding field of data analysis using information gathered
from the genome projects
a. proteomics
b. nutriomics
c. bioinformatics
d. genomics
16. The butterfly and the caterpillar from which it emerges have the same
a. phenotype
b. genotype
c. expression levels of all genes
d. number of chromosomes
e. both b and d
f. both a and c
17. For effective gene therapy, the gene product must be
a. effective at low doses
b. toxic at high doses
c. non-effective at low doses
d. non-toxic at high doses
e. both a and c
f. both a and d
18. Tumor suppressor genes encode proteins that
a. promote tumor growth
b. suppress genes that can lead to tumor formation
c. have been identified using reverse genetics
d. both a and b
e. both b and c

19. The promoter region of a gene
a. is transcribed into RNA
b. promotes transcription
c. promotes termination
d. is in front of the gene to be transcribed
e. is in back of the gene to be transcribed
f. both a and d
g. both b and d
20. Primordial germ cells can give rise to
a. the next generation of sex cells
b. satellite DNA
c. inner mass cells
d. trophoblastic cells
21. One of these is NOT true of adult stem cells
a. are found in very low numbers
b. can be made to differentiate similarly to ES cells
c. die if not surrounded by a placenta
d. have a normal karyotype
e. can be studied using Federal funds
f. X inactivation doesn’t occur
22. Gestation diabetes
a. develops in a small percentage of all pregnant women
b. is associated in women with a family history of diabetes
c. is associated with babies that have elevated glucose levels
d. all of the above
e. only a and b
23. These complications can (and often do) arise from having diabetes
a. eye problems leading to blindness
b. heart disease and poor circulation
c. hair loss
d. increased chance of developing cancer
e. both a and b
f. both b and d
24. Telomeric DNA
a. is found at the ends of chromosomes
b. is always expressed
c. shortens in length as you age
d. makes cells stick together
e. both a and c
f. both b and c

Fill in the blank (2 pts./question;18 pts. total)
_________1.
The first successful gene therapy case in the country cured
what disease?
_________2.
Genetic map unit whereby there is 1% recombination
_________3.
_________4.
_________5.
_________6.
Transgenic fish have been developed to express this protein so
that they can survive the below freezing waters around
Newfoundland.
The first successfully cloned animal by somatic cell nuclear
transfer
ES cells are derived from these blastocyst-derived cells
The worldwide spread of a disease affecting millions of people
_________7.
60%-80% of all tumors are generally derived from this cell type
_________8.
Currently the most rapidly increasing type of diabetes
_________9.
A reason that the private human genome sequencing project
moved so fast

Matching (2pts/question; 18 pts. total).
_____1. These animals don’t appear to get cancer
_____2. A polygenic disease
_____3. A biopharmed product synthesized in goat’s milk
_____4. Skin is derived from this germ cell layer
_____5. Gene therapy on non-germ cells
_____6. A DNA marker
_____7. Important for finding genes by reverse genetics
_____8. Transplanting a foreign organ or tissue into a different species
_____9. Adding a sugar or phosphate group to achieve biological activity
A. Mesoderm K. Cystic Fibrosis
B. Sharks L. 1 antitrypsin for emphysema
C. Post-transcriptional modifications M. Ectoderm
D. Somatic cell therapy N. Jets
E. Diabetes O. SNPs
F. Eugenics P. Reference families
G. Lauraderm Q. Endoderm
H. Xenotransplantation R. Rats
I. Enzymes S. Post-translational modifications
J. Cream cheese T. Huntington’s chorea

Short answer question (6 pts.)
Explain how DNA microarray differential expression analysis of a human
malignant organ tumor can help decide what type of therapy will be used to
combat the disease. Discuss the sequential steps needed to demonstrate this
differential analysis.
Essay question (10 pts.)
Consider the following biomedical ethical dilemma. Even though you’re a really
great person, your immediate family is not so great. Your mother and her father
are convicted felons that have been previously forced to submit to authorities
samples of their own DNA for profiling. Because of public records which your
perspective employer has access to, there is a demand put upon you (but not on
the other five potential new hires) to submit a DNA sample to the company. Who
has ownership of your DNA information? Discuss. Can you file a discrimination
suit against the company for: A) demanding a DNA sample be given based on
your immediate relatives which suggests you might carry similar markers for
being a felon; B) demanding a DNA sample from you but not the five other
potential hires? Discuss in detail. Do you think that it is fair that the company
makes your hiring contingent upon your giving a DNA sample? Why or why not?