(Agri 1501) Recombinant DNA/Animal & Medical Bi - University of ...

Name_____________________________
Biotechnology, Society and the Environment (Agri 1501)
Recombinant DNA/Animal & Medical Biotechnology Exam—March 8, 2006
Multiple Choice. Choose the best answer for each of the following questions.
(2 pts./question; 48 pts. total).
1. A promoter is:
a. a protein
b. an antibody
c. a regulator of RNA transcription in a tissue-specific manner
d. something that binds antigens
2. Pre-implantation diagnosis utilizes
a. PCR analysis to screen for genetic defects
b. in vivo fertilization
c. animal cell transformation
d. repeated, non-expressed sequences
3. The ploidy of a normal somatic animal cell is:
a. 4N
b. 1N
c. 2N
d. sub-tetraploid
e. anueploid
4. Tumor suppressor genes allow:
a. sterility to occur
b. cells to proliferate unregulated
c. oncogenes and cell cycle regulation to function normally
d. are always mutated
e. kill cells
5. A physical method of gene transfer in animal cells:
a. retrovirus
b. herpes virus
c. liposomes
d. microinjection
6. Somatic cell nuclear transfer can produce clones from:
a. nuclei from adult cells
b. tissue DNA
c. mitochondria
d. ribosomes

7. Classical breeding can be used for animal improvement by selecting for:
a. totipotency
b. decreased carcass fat
c. single genes
d. smarter animals
e. smellier animals
8. A disease that worsens as the number of triple nucleotide repeats
increases:
a. Huntington’s chorea
b. Parkinsons
c. diabetes
d. aging
9. What advantages do recombinate proteins made in animal cells have over
those made in bacterial expression systems?
a. they are correctly post-transcriptionally modified
b. they are correctly post-translationally modified
c. they have more amino acids
d. they have fewer amino acids
e. they mutate less frequently
10. Retroviruses spread throughout a brain tumor but not brain nerve cells
because retroviruses target:
a. dividing cells
b. non-dividing cells
c. other viruses
d. bacteria
e. only cerebral brain cells
11. Currently the most rapidly increasing type of diabetes
a. Type 1
b. Type 2
c. Type 3
d. Gestational
12. Microinjection of animal embryos works with low efficiency, but not at all
for:
a. pigs
b. chickens
c. cattle
d. mice

13. The way to tell if a transgene construct is actually expressed
a. northern blot analysis
b. Southern blot analysis
c. western blot analysis
d. eastern blot analysis
14. Example of an ectodermal cell type:
a. Skin
b. Bone
c. Liver
d. Muscle
15. A biological reason NOT to clone humans:
a. can’t be 100% sure all genes will be expressed normally
b. they won’t all look like each other
c. they won’t all be the same size
d. they won’t have the same intelligence
16. Embryonic stem cells derived from the inner cell mass of the blastoderm
that give rise to the gonads and the next generation of sex cells
a. STRs
b. PGCs
c. ESTs
d. SNPs
17. Altered nuclear transfer (ANT) is a very new technology that gets around
destroying embryos to derive ES cells and works
a. just like somatic cell nuclear transfer
b. by using somatic cell nuclear transfer with the gene for implantation
into the uterus knocked out
c. just like therapeutic cloning
d. just like reproductive cloning
e. probably won’t work
18. Replacement proteins produced following gene therapy should:
a. be toxic only at high doses
b. be effective at less that 100% normal values
c. be toxic to cure the disease
d. be able not to cure the disease at low doses

19. The new discipline of analyzing data from the genome projects
a. bioinformatics
b. hyper genomics
c. nutriomics
d. proteomics
e. secretomics
20. A relative map distance where there is 1% recombination
a. is a physical measurement
b. is a genetic measurement called a centimorgan
c. is the same in all species
d. is a way to measure repetitive DNA
21. The CFTR and Huntington’s genes were both isolated using
a. forward genetics
b. reverse genetics
c. library screeing
d. enzymatic activity
e. biochemical analysis
22. Epithelial cells
a. look just like fibroblastic cells
b. have a different karyotype than fibroblastic cells
c. are cobblestone in appearance and are the origins of about 60% of all
human tumors
d. cannot be grown using cell culture methods
23. Embryonic stem cells when inside a trophoblast are considered to be:
a. pluripotent
b. impotent
c. totipotent
d. omnipotent
e. awful
24. The newly completed Zebrafish genome project is important for:
a. Repetitive elements
b. Functional genomics
c. RFLP analysis
d. bait

Fill in the blank (2 pts./question;18 pts. total)
_________1. In the presence of “Blue-Gal”, white colonies are bacteria that
contain: A) Non-recombinant plamids; or B) Recombinant
plasmids
_________2.
Type of enzyme that recognizes specific DNA sequences and
cut at that site.
_________3.
_________4.
_________5.
_________6.
Which type of diabetes is the result of autoimmunity?
What a foreign organ or tissue transplanted into a different
species is called
Inner mass cells can give rise to these pluripotent cells grown in
culture
Name an autosomal recessive disease
_________7.
Mitochondrial DNA can be used to detect maternally inherited DNA
but can’t discern brother/sister mitochondrial DNA (T/F?).
_________8.
Three generations (or more) of ________ families that have a disease
are used in reverse genetics to locate disease genes.
_________9.
If a DNA polymorphism is very close to a gene that causes a
disease, it can be used as a reliable_________for that disease.

Matching (2pts/question; 18 pts. total).
_____1. Way to prove a transgene was injected into a mouse fertilized pronuclei
_____2. An example of a polygenic trait
_____3. Transgenic fish with this protein can grow in very cold (28 o F) waters
_____4. Adenovirus vectors are used in cystic fibrosis gene therapy because
they “home” to this organ.
_____5. The “H” in avian influenza H5N1 stands for what?
_____6. Researchers at the University of Minnesota have just recently shown
that pancreatic islet cells transplanted from these animals produce
insulin in monkeys.
_____7. In the PCR reaction, what step occurs at about 55-62 o C?
_____8. Chromosomal genetic disorder
_____9. Research method where expression levels of all the genes in an
organism can be analyzed at one time
A. Hypertension K. Antifreeze protein
B. Foot L. Hemagglutinin
C. Northern blot M. Left handedness
D. Post transcriptional N. Primer annealing
E. Eastern blot O. Totipotent
F. Herpes P. Lung
G. Zebrafish Q. Trisomy 21 (Down’s syndrome)
H. ADA R. DNA microarray chip
I. Denaturing S. Southern blot
J. Microwave T. Pigs

Short answer question (6 pts.)
Using nuclear DNA, short tandem repeats can be used to identify 1 person out of
10 18 . But only 13 different primer pairs are used to identify these differences.
How can so few different primer pairs identify a single genotype (person) out of
such a huge human genotype (or population of10 18 )?
Essay question (10 pts.)
Given our current national debate over civil liberties vs. national security and the
wire-tapping of private citizens, explain how you feel about the potential use in
the future of your genetic code by the government. How do you feel about the
government using your genetic information to alert you to a horrible genetic
disorder. Is your opinion different if your doctor alerts you? How so? What if the
government uses genetic information to alert your community about a potential
sociopath based on an identified genetic mutation? Are your answers to all of
these questions consistent? Explain why or why not.