Enzyme Engineering (pdf)
Enzyme Engineering (pdf)
Enzyme Engineering (pdf)
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
Genes Modified by DNA Shuffling<br />
• Glyphosphate – active ingredient in Roundup<br />
• Bacteria were screened for the ability to detoxify glyphosphate<br />
• The relevant genes were isolated<br />
• DNA shuffling was used to increase glyphosphate tolerance by<br />
FOUR ORDERS OF MAGNITUDE<br />
• The “new and improved” enzyme was used to develop<br />
Roundup resistant crops<br />
Review Questions<br />
• Terms: enzyme, activation energy, active site, access<br />
tunnel, specificity, substrate, product, immobilized<br />
enzyme<br />
• What advantages do enzymes have over live cells for<br />
industrial applications?<br />
• What benefits do immobilized enzymes offer over<br />
free enzymes for use in industrial processes?<br />
• What is bioprospecting?<br />
• Distinguish between DNA shuffling and site-directed<br />
mutagenesis.
Change language