Enzyme Engineering (pdf)
Enzyme Engineering (pdf)
Enzyme Engineering (pdf)
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<strong>Enzyme</strong> modified by site-directed<br />
mutagenesis<br />
• Trichloropropane (TCP) is a carcinogenic compound<br />
that is persistent in the environment<br />
• Researchers began with an enzyme that could<br />
degrade TCP<br />
• They created mutants by replacing amino acids in<br />
the enzyme’s access tunnel<br />
• The “new and improved” enzyme was able to break<br />
down trichloropropane (TCP) with 32 times greater<br />
activity than the wild type enzyme<br />
DNA Shuffling<br />
• Recombining fragments of multiple genes<br />
encoding the same enzyme<br />
• Improve enzyme function<br />
DNA Shuffling<br />
1. Parent genes – slightly different<br />
genes that encode same product<br />
2. Genes chopped into random<br />
fragments<br />
3. Gene fragments recombined<br />
via PCR<br />
4. DNA shuffling results in<br />
unique, full-length genes