Enzyme Engineering (pdf)
Enzyme Engineering (pdf)
Enzyme Engineering (pdf)
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Optimizing <strong>Enzyme</strong>s Function<br />
• Parameters to optimize:<br />
• Strategies for optimization:<br />
–Bioprospecting<br />
–Directed evolution<br />
Bioprospecting<br />
• Search for organisms living in natural<br />
environments (especially extreme<br />
environments) to identify unique enzymes.<br />
Applications of enzymes<br />
discovered by bioprospecting<br />
• Lactase from cold-loving bacteria is used in<br />
the dairy industry<br />
• Taq polymerase from heat-loving bacteria is<br />
used in PCR
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