10 A niversary of IIMCB
10 A niversary of IIMCB
10 A niversary of IIMCB
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Marcin Nowotny,<br />
PhD<br />
DEGREES<br />
• PhD magna cum laude in biochemistry, Nencki Institute <strong>of</strong><br />
Experimental Biology PAN, Department <strong>of</strong> Molecular and<br />
Cellular Neurobiology, Warsaw, 2002<br />
• MSc in organic chemistry and biochemistry, Warsaw<br />
University, Department <strong>of</strong> Chemistry 1998<br />
POSTDOCTORAL TRAINING<br />
2003-2008 Postdoctoral Fellow, Wei Yang laboratory,<br />
National Institutes <strong>of</strong> Health, National Institute<br />
<strong>of</strong> Diabetes & Digestive & Kidney Diseases,<br />
Bethesda MD, USA<br />
PROFESSIONAL EMPLOyMENT<br />
Since 2008 Head <strong>of</strong> the Laboratory <strong>of</strong> Protein Structure,<br />
<strong>IIMCB</strong><br />
HONORS, PRIZES, AWARDS<br />
2003 Prime Minister’s award for PhD thesis<br />
2001, 2002 Annual Stipend for Young Scientists <strong>of</strong> the<br />
Foundation for Polish Science<br />
1999 Fellowship <strong>of</strong> the Kronenberg Bank Foundation<br />
74 Annual Report 2008<br />
Selected publications<br />
• Nowotny M. Retroviral integrase superfamily: the<br />
structural perspective (review). EMBO Rep, 2009; <strong>10</strong>:144-51<br />
• *Nowotny M, Cerritelli SM, Ghirlando R, Gaidamakov<br />
SA, Crouch RJ, Yang W. Specific recognition <strong>of</strong> RNA/DNA<br />
hybrid and enhancement <strong>of</strong> human RNase H1 activity by<br />
HBD. EMBO J, 2008; 27:1172-81<br />
• *Nowotny M, Gaidamakov SA, Ghirlando R, Cerritelli<br />
SM, Crouch RJ, Yang W. Structure <strong>of</strong> human RNase H1<br />
complexed with an RNA/DNA hybrid: Insight into HIV<br />
Reverse Transcription. Mol Cell, 2007; 28:264-276<br />
• *Nowotny M, Yang W. Stepwise analyses <strong>of</strong> metal Ions<br />
in RNase H catalysis: From substrate destabilization to<br />
product release. EMBO J, 2006; 25:1924-33<br />
• *Yang W, Lee JY, Nowotny M. Making and Breaking<br />
Nucleic Acids: Two-Mg 2+ -ion Catalysis and Substrate<br />
Specificity, (review). Mol Cell, 2006; 22:5-13<br />
• *Nowotny M, Gaidamakov SA, Crouch RJ, Yang W. Crystal<br />
structures <strong>of</strong> RNase H bound to an RNA/DNA hybrid:<br />
substrate specificity and metal-dependent catalysis. Cell,<br />
2005; 121:<strong>10</strong>05-16<br />
• *Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J,<br />
Chazin WJ. Human Sgt1 binds HSP90 through the CHORD-<br />
Sgt1 domain and not the tetratricopeptide repeat domain.<br />
J Biol Chem, 2004; 279:16511-7<br />
• *Nowotny M, Spiechowicz M, Jastrzebska B, Filipek A,<br />
Kitagawa K, Kuznicki J. Calcium-regulated interaction <strong>of</strong><br />
Sgt1 with S<strong>10</strong>0A6 (calcyclin) and other S<strong>10</strong>0 proteins. J Biol<br />
Chem, 2003; 278:26923-8<br />
• *Filipek A, Jastrzebska B, Nowotny M, Kuznicki J. CacyBP/<br />
SIP, a calcyclin and Siah-1-interacting protein, binds EFhand<br />
proteins <strong>of</strong> the S<strong>10</strong>0 family. J Biol Chem, 2002;<br />
277:28848-52<br />
• *Filipek A, Jastrzebska B, Nowotny M, Kwiatkowska<br />
K, Hetman M, Surmacz L, Wyroba E, Kuznicki J. Ca 2+ -<br />
dependent translocation <strong>of</strong> the calcyclin-binding protein<br />
in neurons and neuroblastoma NB-2a cells. J Biol Chem,<br />
2002; 277:21<strong>10</strong>3-9<br />
• *Nowotny M, Bhattacharya S, Filipek A, Krezel AM,<br />
Chazin W, Kuznicki J. Characterization <strong>of</strong> the interaction<br />
<strong>of</strong> calcyclin (S<strong>10</strong>0A6) and calcyclin-binding protein. J Biol<br />
Chem, 2000; 275:31178-82.<br />
*Papers marked with an asterisk have no the <strong>IIMCB</strong> affiliation<br />
<strong>of</strong> the authors<br />
Description <strong>of</strong> Current<br />
Research<br />
Our laboratory focuses on structural and biochemical<br />
studies <strong>of</strong> nucleic acid enzymes. Our primary method is<br />
protein crystallography. Our projects can be subdivided into<br />
three groups:<br />
1. Structural studies <strong>of</strong> substrate complexes <strong>of</strong> members <strong>of</strong><br />
integrase superfamily.<br />
2. Structural studies <strong>of</strong> reverse transcriptases.<br />
3. Structural studies <strong>of</strong> UvrA DNA repair protein.