ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

e used to cultivate endothelial cells in order to induce phenotype similar to in vivo
conditions in blood vessels.
Acknowledgements
This work was supported by the European Social Fund - Project OrganoNET
(CZ.1.07/2.4.00/31.0245) and HistoPARK (CZ.1.07/2.3.00/20.0185) and project FNUSA-
ICRC (CZ.1.05/1.1.00/02.0123).
References
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P46. DEVELOPMENT OF EUROFLOW STANDARDIZED PROTOCOL FOR DIAGNOSTIC
EVALUATION OF PRIMARY IMMUNODEFICIENCY
Tomáš Kalina 1 , Vendula Šinkorová 1 , Ester Mejstříková 1 , Michaela Nováková 1 , Marcela
Vlková 2 , Menno C. van Zelm 3 , Martin Perez-Andres 4 , Eduardo Lopez 5 , Alberto Orfao 4 ,
Jacques J. M. van Dongen 3 , Mirjam van der Burg 3
1
Pediatric Hematology and Oncology, Charles University Prague, 2 nd Medical Faculty,
Praha 5, Czech Republic,
2
Department of Clinical Immunology and Allergology, St. Anne’s University Hospital and
Faculty of Medicine, Masaryk University, Brno, Czech Republic
3
Immunology Department, Erasmus MC, Rotterdam, The Netherlands
4
Cancer Research Center (IBMCC-CSIC), Department of Medicine and Cytometry Service,
University of Salamanca, Salamanca, Spain
5
Clinical Immunology Service, Hospital La Paz, Madrid, Spain
Primary immunodeficiency (PID) is an immune system malfunction caused by hereditary
mutation of genes encoding proteins which are involved in differentiation or effector
functions of immune cells or immunodeficiency with unknown cause (not related to
infection or therapy) . Flow cytometric immunophenotyping of peripheral blood and
bone marrow has become central in diagnostics and research on PID. Many centers have
developed multi-color flowcytometric protocols, but differences in antibody panels,
sample handling, instrument setup and data analysis hamper exchange of data between
centers and understanding of rare cases.
The goal of EuroFlow PID project was to develop standardized 8-color immunophenotyping
panel for initial screening of patients suspected with immunodeficiency. First tube (so
called “screening tube”) of this panel allows for analysis of all main lymphocyte subsets
(T, B and NK cells) in one tube and their maturational status. Standardized, multi centric
approach facilitates joint data analysis using novel data analysis tools (Infinicyt software).
142 Analytical Cytometry VII