ABSTRACTS – ORAL PRESENTATIONS - AMCA, spol. s r.o.

this process. Combined treatment with TRAIL and DHA markedly stimulated apoptosis
in SW620 cells, which included the activation of proapoptotic Bcl-2 family proteins Bak
and Bax, facilitated permeabilization of the outer mitochondrial membrane and release
of cytochrome c, decrease in mitochondrial membrane potential and inhibition of
mitochondrial respiration.
In summary, our results show that DHA can be used to enhance TRAIL-induced apoptosis
in resistant colon cancer cells by targeting mitochondria.
This work was supported by grants Nos. P301/11/1730, 13-097665 of the Czech Science
Foundation and MSM0021622430 Ministry of Education, Youth and Sports.
P28. ANALYSIS OF MIGRATION AND INVASION PROPERTIES ASSOCIATED WITH
CANCER TRANSFORMATION OF HUMAN EPITHELIAL CELLS
Eva Slabáková 1,2 , Veronika Toporcerová 1,2,3 , Radek Fedr 1 , Antonín Hlaváček 4 , Petr
Skládal 4 , Alois Kozubík 1,3 , Karel Souček 1,2
1
Academy of Science of the Czech Republic, Institute of Biophysics, Department of
Cytokinetics, Královopolská 135, 61265 Brno, Czech Republic
2
International Clinical Research Center, St. Anne‘s University Hospital Brno, Brno, Czech
Republic
3
Department of Experimental Biology, Masaryk University, Faculty of Science, Brno,
Czech Republic
4
Centre for Structural Biology, Central-European Technology Institute, Brno, Czech
Republic.
Correspondence to: slabakova@ibp.cz
Most cancer–related deaths are associated with advanced disease and metastasis.
Epithelial-mesenchymal transition (EMT) facilitates cancer cell dissemination and
metastasis by changes in cell polarity, cell-cell adhesions and organization of cytoskeleton.
Moreover, EMT can also facilitate survival of metastasizing cells by endowing them with
cancer stem cell properties.
Cell adhesion, interaction with extracellular matrix (ECM) and reorganization of
cytoskeleton are crucial processes implicated in cell migration and invasion. While
certain cell types invade through the ECM using extracellular protein degradation by
enzymatic activity of matrix metalloproteases (MMP), other cell types rely on their
ability to reorganize the cytoskeleton and are characterized by an amoeboid mode of
migration which does not involve ECM degradation.
Analyzing paired benign and tumorigenic cell lines derived from benign human prostate
or breast tissue, we observed that cancer transformation is often accompanied by EMT,
as evidenced by decreased expression of epithelial markers and increased expression of
mesenchymal markers and EMT regulating transcription factors. To confirm that these
changes in gene and protein expression and localization are functionally implicated in cell
motility, we compared the migration and invasion potential of benign and transformed
120 Analytical Cytometry VII